心理社会压力对喉微生物学和上皮质屏障完整性的影响
Anumitha Venkatraman1, Katelyn Jacobs1, John Binns1
1Department of Otolaryngology - Head & Neck Surgery, University of Wisconsin-Madison, Madison, USA.
Scientific reports
|July 12, 2025
概括
心理社会压力显著改变喉,影响微生物多样性和声上皮质完整性,特别是在女性中. 长时间的压力重塑了喉微生物组,并削弱了声屏障.
科学领域:
- 微生物学 微生物学
- 身体生理学 身体生理学
- 压力研究 压力研究
背景情况:
- 众所周知,心理社会压力会影响喉生理.
- 将压力与喉变化联系在一起的特定生物机制仍未得到充分研究.
- 了解这些机制对于语音健康至关重要.
研究的目的:
- 研究心理社会压力对喉微生物群组成的影响.
- 评估压力对声上皮质完整性的影响.
- 要确定压力持续时间和性别是否影响这些喉变化.
主要方法:
- 108只C56BL/7小鼠受到短期 (7天) 或长期 (14天) 的约束压力.
- 使用16S rRNA测序分析了喉微生物群的组成.
- 声上皮质完整性通过RT-qPCR和免疫光检测进行了评估.
主要成果:
- 长时间的压力,与短时间的压力不同,改变了喉微生物的多样性和组成,转向了Firmicutes.
- 压力减少了Corynebacterium的相对丰富性,并在喉中增加了链球菌.
- 压力降低了声表皮中的炎症和与屏障相关的基因/蛋白质 (例如,E-cadherin,Zo-1,MUC2),女性的效果更大,长时间的压力更大.
结论:
- 短期和长期的心理社会压力会改变喉微生物群,并损害声上皮质屏障的完整性.
- 这些影响在女性中更为明显,压力持续时间更长.
- 需要进一步的研究来探索在压力下喉的宿主上皮层-微生物组相互作用.
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