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相关概念视频

RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Sep 15, 2025

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
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Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

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转录组测序揭示了人类精子样本中显著的RNA变异.

Weiming Chen1,2, Lei Yu3,4, Zhenyu Jia5,6

  • 1Guizhou university medical school, South Jiaxiu Rd, Guiyang city, Guizhou province, China.

BMC research notes
|July 12, 2025
PubMed
概括

研究精子RNA概况对于了解男性不孕症和表观遗传影响至关重要. 这项研究提供了有价值的转录组数据,以推进该领域的研究.

关键词:
阿斯诺精子缺陷症 (Asthenospermia) 是一种在RNA-seqqq.精子 精子 精子

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RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
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RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

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Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
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Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

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相关实验视频

Last Updated: Sep 15, 2025

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
08:30

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Published on: December 8, 2021

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RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
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RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse

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Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
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Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

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科学领域:

  • 生殖生物学 生殖生物学
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 精子RNA档案对于了解男性生育能力和表观遗传至关重要.
  • 在精子生物学中RNA数量和质量的作用仍然不太清楚.
  • 研究精子RNA可以揭示与RNA疾病相关的男性不孕症.

研究的目的:

  • 分析精子细胞的转录组从男性与正常和减少的精子运动.
  • 评估精子样本中的RNA完整性和数量.
  • 让有价值的RNA测序数据公开用于进一步研究.

主要方法:

  • 采集了83个精液样本,包括那些患有精 (精子运动性降低) 的精液样本.
  • 在37个成功处理的样本上进行了转录基因组测序.
  • 基于RNA完整数 (RIN) 和28S/18S比率的分类样本.

主要成果:

  • 从37个精液样本中成功对RNA进行了测序.
  • 确定了15个高质量样本 (RIN > 6) 和22个中等质量的样本 (RIN 5-6).
  • 在GSA-Human数据库中公开存储的RNA测序数据 (加入号码HRA006250,HRA006906).

结论:

  • 这项研究提供了关键的,公开可访问的精子RNA测序数据.
  • 这些数据可以帮助开发用于分析精子生成后RNA的算法.
  • 突出了发现精子症的新分子标记物的潜力.