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细菌性阴道炎毒素会损害精子容量和受精能力

Shweta Bhagwat¹, Leila Asadi¹, Ronald McCarthy¹

¹Department of Obstetrics and Gynecology, Washington University School of Medicine, Saint Louis, MO, USA.

Human reproduction (Oxford, England)

|July 13, 2025

概括

细菌性阴道炎 (BV) 毒素,脂多糖 (LPS) 和阴道素 (VLY),损害精子功能. 这些毒素破坏精子电容和水平,降低精子受精卵的能力,并导致不孕.

科学领域:

生殖生物学和毒理学.
微生物病原和不孕症的发生.

背景情况:

细菌性阴道炎 (BV) 与不孕症有关,其特点是Prevotella和Gardnerella等细菌的过度生长.
在BV中存在高度的细菌毒素,包括脂多糖 (LPS) 和阴道素 (VLY),在BV中存在.
这些特定的BV毒素对精子功能和受精能力的影响仍然不完全理解.

研究的目的:

研究与细菌性阴道炎 (BV) 相关的毒素,脂多糖 (LPS) 和阴道素 (VLY) 对人类和小鼠精子功能的影响.
为了确定这些毒素如何影响精子运动,电容,体外细胞形成和细胞内水平.
阐明BV毒素可能导致不孕症的机制.

主要方法:

人类和小鼠的精子被分离出来,在培养和非培养条件下暴露在纯化的LPS和VLY中.
用计算机辅助的精子分析和特定的染色技术来评估精子的运动性,过度激活,活力和体外细胞形成.
使用光显微镜测量细胞内度,并研究了CatSper通道和Toll-like受体4 (TLR4) 的作用.

主要成果:

暴露于LPS和VLY显著降低了小鼠精子中的体外受精 (IVF) 率.
两种毒素最初都增加了,然后显著降低了精子过度活化和运动性,同时在两种物种中减少了体外细胞形成.
这些影响与细胞内的快速,不可逆转的增加有关,LPS的TLR4介导,需要外部,但不是CatSper.

结论:

与BV相关的毒素,LPS和VLY,直接损害了受精所需的关键精子功能.
这些毒素破坏了精子电容和细胞内平衡的调节,最终降低了精子的受精能力.
这些发现表明,BV毒素是通过损害精子功能的不孕症的一个因素.

关键词:

在CatSper上使用.这是一种宏体外细胞形成.这是细菌性阴道炎.过度活化过度活化不孕症不孕不育不孕不育不孕不育脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.精子容量化能力精子容量化能力收费类受体4的收费类受体阴道溶解素 (Vaginolysin) 是一种

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