响应JA的R2R3型MYB转录因子OsMYB4P赋予了大米的广泛抗病毒免疫力
Mingmin Lu1, Qianqian He1, Guoda Wang1
1State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Plant biotechnology journal
|July 13, 2025
概括
米的抗病毒防御包括莉酸 (JA) 信号传输. 一种新的R2R3型MYB转录因子OsMYB4P通过与JAZ蛋白相互作用,激活JA合成并增强对病毒感染的抵抗力.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 生物化学 生物化学
背景情况:
- 雅斯蒙酸 (JA) 信号传递对于米抗病毒防御至关重要.
- JA信号涉及降解雅斯蒙酸ZIM域 (JAZ) 蛋白质以释放转录因子 (TF).
- 在大米中JA信号传递中MYBTFs的作用基本上是未知的.
研究的目的:
- 在大米中识别和表征新的JA-响应的MYB TFs.
- 研究OsMYB4P在JA信号和抗病毒防御中的功能.
- 阐明病毒干扰OsMYB4P功能的机制.
主要方法:
- 对OsMYB4P的基因克隆和表征.
- 对OsMYB4P转录活性和蛋白质相互作用的分析.
- 产生和分析OsMYB4P过度表达和淘汰线.
- 使用南方大米黑条纹矮人病毒 (SRBSDV) 和大米条纹病毒 (RSV) 进行病毒挑战测试.
主要成果:
- OsMYB4P是一种新的JA-响应R2R3型MYB TF,可激活CM-LOX1表达并增强JA灵敏度.
- OsMYB4P与OsJAZs相互作用,并受到它们的负面调节.
- 过度表达OsMYB4P会增强对病毒感染的抵抗力,而淘汰突变更容易受到感染.
- 病毒蛋白SP8和P2破坏了OsMYB4P的自我相互作用,降低了它的稳定性和功能,从而抑制了JA信号.
结论:
- OsMYB4P作为JA信号传递中的转录激活剂,在米抗病毒防御中起着重要作用.
- OsMYB4P是JAZs的直接目标,其功能被病毒蛋白抑制.
- OsMYB4P有助于大米的广泛抗病毒免疫力.
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