相关实验视频
Updated: Sep 15, 2025

12:30
Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
Published on: May 19, 2017
15.0K
通过Ca2+ BmSERCA介导的细胞增殖的机制 恒温调节 调节
Xiaoxia Zhang1, Jie Tang1, Peilin Peng1
1School of Life Sciences, Suzhou Medical College of Soochow University, Suzhou, China.
Archives of insect biochemistry and physiology
|July 13, 2025
概括
干扰Bombyx mori细胞中 Sarcoplasmic/endoplasmic网膜 ATPase (SERCA) 的干扰破坏了平衡,显著加速了细胞的增殖和迁移. 这表明SERCA在调节细胞分裂方面发挥着关键作用.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 昆虫生理学 昆虫生理学
背景情况:
- ATPase (SERCA) 对于维持细胞内 (Ca2+) 恒温至关重要.
- 离子对于Bombyx mori的许多生物过程至关重要,包括发育和繁殖.
研究的目的:
- 研究BmSERCA在Bombyx mori卵巢细胞系 (BmN) 的增殖中的作用.
主要方法:
- 与 BmSERCA 表达式的干扰.
- 测量细胞内Ca2+水平.
- 对与细胞增殖相关的基因转录水平的分析.
- 表型评估包括细胞增殖和迁移测定 (EdU染色).
- 流细胞计分析细胞周期分布和细胞周期调节者的表达 (BmCyclinB,BmCDK1).
主要成果:
- BmSERCA干扰导致细胞内Ca2+水平显著增加 (48小时后是1.38倍).
- 细胞增殖和分裂基因的转录水平显著改变.
- BmSERCA干扰加速了BmN细胞的增殖和迁移,由增加的EDU阳性细胞证明.
- 细胞周期分析显示G2/M相积和BmCyclinB和BmCDK1.1的表达升高.
结论:
- 通过BmSERCA干扰破坏细胞内Ca2+稳态,促进了BmN细胞的增殖.
- BmSERCA在调节BmN细胞增殖方面发挥着重要作用,可能通过调节细胞周期.
- 这些发现为了解SERCA在昆虫细胞增殖中的作用提供了基础.
相关概念视频
Feedback Regulation of Calcium Concentration
3.5K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.5K
Calmodulin-dependent Signaling
5.3K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.3K
MAPK Signaling Cascades
6.1K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
6.1K
Mitogens and the Cell Cycle
6.9K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.9K
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
Skeleton and Calcium Homeostasis
4.8K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
4.8K

