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单细胞RNA测序研究揭示了RPS26基因在注意力缺陷多动性障碍中的潜在作用

Sung-Chou Li¹, Liang-Jen Wang², Ho-Chang Kuo³

¹Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan; Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan.

Progress in neuro-psychopharmacology & biological psychiatry

|July 13, 2025

概括

研究人员发现,注意力缺陷/多动症障碍 (ADHD) 患者的RPS26基因表达减少. 这可能解释了与ADHD相关的心血管风险增加,因为它会影响单细胞的发育和功能.

科学领域:

神经发育障碍神经发育障碍
免疫学免疫学免疫学
心血管疾病是什么心血管疾病

背景情况:

注意缺陷/多动障碍 (ADHD) 是一种常见的神经发育障碍.
现有的ADHD基因表达研究受限于大量的RNA分析,掩盖了细胞类型特定的见解.
ADHD与心血管疾病风险增加有关,涉及单细胞和巨细胞.

研究的目的:

通过专注于单细胞/巨细胞生物学来研究ADHD和心血管疾病之间的潜在联系.
通过单细胞RNA测序,识别免疫细胞中与ADHD相关的基因表达变化.

主要方法:

单细胞RNA测序 (scRNA-seq) 的白细胞 (WBCs) 来自双胞胎双胞胎不一致的ADHD.
定量PCR (qPCR) 用于基因表达的验证.
在体外测试以评估RPS26在单细胞中的功能影响.

主要成果:

确定了12种不同的免疫细胞类型和差异表达的基因,包括单细胞中的RPS26.
在ADHD患者的白细胞和单细胞中证实显著降低RPS26表达.
RPS26的淘汰抑制了单细胞增殖,促进了细胞亡,降低了分化能力,改变了RNA比率.

结论:

RPS26在单细胞的发育和分化中起着调节作用.
减少RPS26表达可能导致ADHD患者心血管风险升高.
这项研究为ADHD和心血管疾病之间的潜在分子联系提供了新的见解.

关键词:

更多关于 ADHD ADHD 的文章细胞灭亡 (apoptosis) 是一种死亡的过程.细胞的增殖细胞的增殖.不同化的差异化巨细胞是一个巨细胞.一个单细胞单细胞.在 RPS2626 中使用.单细胞RNA测序的一个细胞.

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相关概念视频

Attention-Deficit/Hyperactivity Disorder

Attention-Deficit/Hyperactivity Disorder

Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder characterized by persistent inattention, hyperactivity, and impulsivity. It affects approximately 5-8% of children globally, with around 60-70% of cases persisting into adulthood. ADHD has significant implications for educational attainment, social interactions, and occupational success.
Diagnostic Criteria and Symptoms
To diagnose ADHD, symptoms must manifest before age 12 and be evident across multiple settings....

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...