通过双模式ICP-MS在全血和尿液中同时测定多元元素
Yan Li1, Jian-Yuan Zhong2, Ya-Qi Mo2
1Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease, Nanning, 530021, China.
Analytical biochemistry
|July 13, 2025
概括
这项研究引入了一种双模式ICP-MS方法,用于精确检测血液和尿液中的元素. 这种创新方法准确地量化了22个关键元素在广泛的度范围内,改进了人类健康分析.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 环境健康 环境健康
背景情况:
- 对生物样本进行准确的元素分析对于了解人类健康至关重要.
- 元素度和复杂矩阵的变化带来了重大的分析挑战.
- 现有的方法与广泛的生理学关键元素的动态范围作斗争.
研究的目的:
- 开发和验证一种新的双模式ICP-MS方法论,用于在生物矩阵中超广动态范围的元素量化.
- 为了应对在血液和尿液中检测22个生理关键元素的分析挑战.
- 为了提高与健康相关的研究的多元元素分析的准确性和稳定性.
主要方法:
- 一种双模式的感应合等离子体质谱法 (ICP-MS) 方法,集成动能歧视 (KED) 和动态反应电池 (DRC) 技术.
- 四极排斥参数 (RPa/RPq) 的同步优化以抑制干扰并保持线性探测器响应.
- 双模式分析:KED-He用于尿液和血液中的特定元素;DRC-NH3用于血液中易干扰的元素.
主要成果:
- 成功量化了22个生理关键元素 (例如,Be,V,Cr,Mn,Fe,Ca,Mg,Ba,Co,Cd,Cu,Zn,As,Se,Ti,Sr,Ni,Mo,Sn,Sb,Tl,Pb) 在六个数量级 (μg/L到g/L) 中.
- 达到了0.5-7.2% (血液) 和1.6-5.5% (尿液) 的日内精度,日间变化≤9.6%.
- 证明强大的抑制矩阵衍生的多原子干扰和线性探测器响应.
结论:
- 开发的双模式ICP-MS方法为复杂的生物样本中准确可靠的多元元素分析提供了一个强大的工具.
- 这种方法克服了以前在动态范围和干扰抑制方面的局限性,用于血液和尿液中的元素分析.
- 这些发现支持增强诊断能力和更深入地了解人类健康中的基本作用.
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