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描述压力类似的低剂量皮质醇暴露对人类声纤维细胞的影响
Anumitha Venkatraman1, Wen-Hsuan Tseng2, Susan Thibeault1
1Department of Otolaryngology - Head and Neck Surgery, University of Wisconsin Madison, Madison, WI.
概括
心理社会压力,模拟皮质醇暴露,改变人类声纤维细胞 (hVFF) 基因表达,抑制炎症和促进纤维化. 联合压力和LPS治疗在hVFF中放大了炎症反应和纤维化标志物.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 心理社会压力会增加循环中的皮质糖.
- 压力诱导的葡萄皮质激素对人类声纤维细胞 (hVFF) 的影响尚不清楚.
- 研究这些影响对于理解压力下声健康至关重要.
研究的目的:
- 调查压力相似的皮质醇暴露对hVFF的影响.
- 检查皮质醇和脂多糖 (LPS) 对hVFF的联合作用.
- 为了确定炎症,葡萄糖皮质体信号传递和纤维性基因表达的变化.
主要方法:
- hVFF暴露于皮质醇 (100nM,7天),LPS或两者的组合,以及未经治疗的对照组.
- 对炎症标记物 (IL1-β,TNF-α,TGF-β1),葡萄糖皮质体信号 (11β HSD1,GILZ) 和纤维细胞标记物 (ACTA2) 的相对基因表达量化.
- 使用免疫细胞化学评估了α-SMA (α-光滑肌肉活性蛋白) 蛋白质表达.
主要成果:
- 皮质醇治疗单独提高了GILZ和ACTA2的调节,同时降低了hVFF中的IL1-β的调节.
- 治疗LPS显著增加了炎症性细胞因子基因表达 (IL1-β,TNF-α,TGF-β1).
- 联合皮质醇和LPS治疗可提高IL1-β和α-SMA蛋白表达的调节.
结论:
- 与压力类似的皮质醇暴露通过抑制炎症和促进纤维化基因表达来调节hVFF.
- 皮质醇和LPS的组合增强了hVFF中的炎症反应和纤维化标志物.
- 这些发现提供了关于声对压力的反应背后的细胞机制的见解.
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