影响基于SEM-EDX数据的定量化学分析准确度的决定因素,用于识别自然存在的单个致癌性埃里奥尼特纤维
Wenxia Wendy Fan1, Alessandro F Gualtieri2, Ayrton Hamilton3
1School of Environment, Faculty of Science, University of Auckland, Auckland, New Zealand. wendy.fan@auckland.ac.nz.
Scientific reports
|July 13, 2025
概括
使用扫描电子显微镜与能量分散式X射线分析 (SEM-EDXA) 准确识别致癌物埃里奥尼特是具有挑战性的. 这项研究表明,SEM-EDXA经常对单个石纤维进行不准确的元素分析,因此需要改进公共卫生保护方法.
科学领域:
- 环境科学 环境科学
- 矿物学是什么?矿物学是什么?
- 分析化学 分析化学
- 公共卫生 公共卫生
背景情况:
- 埃里奥尼特是第一组致癌物,作为空气中的可呼吸纤维,对公众健康构成重大风险.
- 扫描电子显微镜与能量分散式X射线分析 (SEM-EDXA) 常用于识别环境样本中的埃里欧尼特纤维.
- 对于单个埃里奥尼特纤维而不是批量样本,SEM-EDXA的定量准确性尚未得到充分证实.
研究的目的:
- 评估SEM-EDXA的可靠性和准确性,用于单个埃里奥尼特纤维的定量化学分析.
- 评估不同样本制备方法对石的SEM-EDXA结果的影响.
- 将SEM-EDXA数据与电子探针微分析 (EPMA) 参考数据进行比较,以评估准确性.
主要方法:
- 使用两种不同的SEM-EDS系统,从高纯度散装样本中分析了325个单独的埃里奥尼特纤维.
- 研究四种常见的样品制备方法,包括脱离离子水分散和过氧化消化.
- 将SEM-EDXA结果与来自大样本的现有EPMA数据进行比较.
主要成果:
- 观察到有系统地高估 (Si) 和 (Mg),低估 (Al), (K) 和 (Ca).
- 在纤维>0.5μm中可靠检测到框架元素 (Si,Al),但制备方法带来了变化.
- 分析的单个纤维中没有一个符合埃里奥尼特识别的定量化学标准.
结论:
- 对于单个埃里奥尼特纤维的定量化学分析,SEM-EDXA的准确性有限,具有显著的元素偏差.
- 样品制备方法可以引入离子交换和离子调动,影响分析结果.
- 提高精度需要使用埃里奥尼特标准进行预校准,应用校正指数,并通过带有选择区域电子衍射 (TEM-SAED) 的传输电子显微镜进行确认.
相关概念视频
01:21Atomic Absorption Spectroscopy: Lab
566
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
566
01:29Atomic Emission Spectroscopy: Lab
249
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
249
01:20Atomic Emission Spectroscopy: Overview
2.6K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
2.6K
01:07Scanning Electron Microscopy
4.4K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fundamental Principles
Accelerated...
4.4K
01:30Atomic Emission Spectroscopy: Interference
281
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
281