在多原子研究中,CXCL14通过NF-κB通路激活驱动与年龄相关的椎间盘退化
Tianle Zhou1, Tonghui Zhang1, Qiwang He1
1College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan, 430061, China.
Scientific reports
|July 13, 2025
概括
椎间盘退化 (IDD) 与慢性腰部疼痛有关. 研究人员发现,CXCL14在老年IDD患者中显著上调,这表明它是治疗年龄相关疼痛的治疗标.
科学领域:
- 生物化学 生物化学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 椎间盘退化 (IDD) 是慢性腰部疼痛的主要原因,严重影响患者的生活质量.
- 现有研究已经确定了IDD的一些生物标志物,但缺乏综合转录基因和蛋白质基因数据用于与年龄相关的变化的全面分析.
研究的目的:
- 整合转录组和蛋白组分析,以确定与年龄相关的IDD相关的关键分子特征.
- 发现改善与年龄相关的IDD治疗的潜在治疗点.
主要方法:
- 在老年和年轻的患有椎间盘病变的患者身上进行了RNA测序和质谱.
- 统计分析,基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 丰富分析被用来解释转录和蛋白质组数据.
- 验证使用外部数据集和RT-qPCR,以及基因调控网络和ceRNA网络分析进行.
主要成果:
- 转录和蛋白质组分析确定了45个不同表达的基因和34个与IDD相关的不同表达的蛋白质.
- 在IDD中,CXCL14是唯一一个在转录基因 (4.2倍) 和蛋白质基因 (3.8倍) 两级显著上调的分子.
- 证实CXCL14在老年IDD患者中过度表达,并与炎症媒介相互作用,激活NF-κB通路.
结论:
- CXCL14是与年龄相关的IDD和相关疼痛的潜在治疗标.
- 这些发现为开发针对性治疗和IDD的诊断工具提供了基础.
- 这项研究为管理与年龄相关的IDD和慢性腰部疼痛的治疗策略提供了见解.
相关概念视频
NF-κB-dependent Signaling Pathway
7.9K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.9K
TGF - β Signaling Pathway
7.7K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.7K
The Effect of Aging on Tissues
2.5K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
2.5K


