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ZmWRKY107通过调节ZmPOD52表达来调节玉米植物的盐分耐受性

Ronghao Cai^1,2, Haidong Yan¹, Xueru Zhu¹

¹National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.

|July 13, 2025

概括

玉米 WRKY 转录因子 ZmWRKY107 通过调节 ZmPOD52 基因表达来增强盐分耐受性. 这一发现为改善玉米耐盐性提供了一个新的候选基因.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

盐度是一个主要的环境压力,影响植物生长.
WRKY转录因子是植物应激反应的关键调节者.
了解玉米 (Zea mays L.) 的盐耐受机制对于农业至关重要.

研究的目的:

调查ZmWRKY107在玉米盐应激反应中的作用.
阐明ZmWRKY107赋予盐分耐受性的分子机制.
确定ZmWRKY107作为增强作物弹性的一种潜在目标.

主要方法:

在盐应激下进行基因表达分析 (RNA-seq,qRT-PCR).
亚细胞局部化和转录活动测试.
酵母1杂交和露西法酶记者测定证实促进体结合.
在盐应力下对wrky107突变体的表型分析.

主要成果:

ZmWRKY107的表达是由盐应力诱导的,它局限于细胞核.
ZmWRKY107特别与ZmPOD52促进体中的W-box元素结合.
wrky107突变体对盐应激的敏感性增加,生理参数发生变化.
ZmWRKY107调节ZmPOD52的表达,有助于盐耐受性.

结论:

ZmWRKY107作为玉米盐应激反应的积极调节者.
ZmWRKY107-ZmPOD52分子模块是玉米盐耐受性的关键组成部分.
ZmWRKY107是开发耐盐玉米品种的有希望的候选基因.

关键词:

在ZmPOD52中,使用的是ZmPOD52.ZmWRKY107 这是一本书.玉米玉米玉米是一种在RNA-seqqq.盐的压力是盐的压力.转录因子 WRKY 转录因子

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