相关实验视频
Updated: Sep 15, 2025

09:23
Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
14.0K
通过MLO介导的Ca2+流入调节了Arabidopsis的根毛尖生长
Sienna T Ogawa1,2, Weiwei Zhang1,2,3, Christopher J Staiger1,2,3
1Department of Botany and Plant Pathology, Purdue University, 915 Mitch Daniels Blvd, West Lafayette, IN, 47907, USA.
The New phytologist
|July 14, 2025
概括
FER/MLO信号模块通过控制和ROS水平来调节根毛发的生长. 这项研究揭示了MLO蛋白调解流入和ROS生产,这对于尖端生长至关重要.
科学领域:
- 植物生物学 植物生物学
- 细胞信号传递 细胞信号传递
- 分子遗传学 分子遗传学
背景情况:
- 根头发的生长需要协调的 (Ca2+) 和反应性氧物种 (ROS) 信号,以延长和完整.
- 温和抗性LOCUS-O (MLO) 蛋白质在FERONIA (FER) 受体类激酶的下游运作,影响花粉管和协同体中的动态.
研究的目的:
- 调查FER/MLO信号模块在根毛尖生长期间调节细胞质Ca2+ ([Ca2+]cyt) 振荡中的新型作用.
- 利用构成性活性MLO (faNTA) 来操纵铁突变体中的Ca2+流入并阐明FER/MLO通路功能.
主要方法:
- 对R-GECO1报告器的光片光成像,以可视化根毛延长期间的[Ca2+]细胞动态.
- 在各种遗传背景中分析根毛发育,[Ca2+]细胞振荡和ROS水平,包括铁突变体和mlo15突变体.
主要成果:
- 构成性活性MLO (faNTA) 恢复了铁-4突变体中的正常根毛发生长,[Ca2+]细胞振荡和ROS水平.
- MLO15被确定为根毛尖生长的关键调节剂,mlo15-4突变体表现出生长中断和改变的[Ca2+]细胞特征.
- FER/MLO模块与ROS积累有关,因为faNTA在fer-4中恢复了ROS水平,但在rbohc突变中没有.
结论:
- MLO蛋白在FER的下游作用,调解Ca2+的流入,促进ROS的产生,以调节根头发的生长.
- 通过协调的Ca2+和ROS信号传输,FER/MLO信号模块对于保持根毛尖生长的完整性至关重要.
相关概念视频
Calmodulin-dependent Signaling
5.3K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.3K
Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K
Morphogenesis
28.8K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.8K
Water and Mineral Acquisition
33.7K
Specialized tissues in plant roots have evolved to capture water, minerals, and some ions from the soil. Roots exhibit a variety of branching patterns that facilitate this process. The outermost root cells have specialized structures called root hairs that increase the root surface, thus increasing soil contact. Water can passively cross into roots, as the concentration of water in the soil is higher than that of the root tissue. Minerals, in contrast, are actively transported into root cells.
33.7K
Responses to Salt Stress
13.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.4K
Regulation of Transpiration by Stomata
29.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
29.1K

