非二甲基连接的二甲基四桑的抗菌活性:对稳定性和有效性的洞察力 针对格拉姆阳性细菌
Guowei Cai1,2, Wenni He1, Xinxin Hu3
1China Pharmaceutical Culture Collection, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
Journal of natural products
|July 14, 2025
概括
阿弗拉桑B是一种真菌化合物,显示出对格拉姆阳性细菌的抗菌活性. 它的转化产品,aflaxanthones C-F,没有表现出类似的疗效,这表明稳定性和抗菌性质之间存在联系.
科学领域:
- 自然产品化学 自然产品化学
- 微生物学 微生物学
- 菌类代谢学 菌类代谢学
背景情况:
- 内生菌是生物活性二次代谢产物的丰富来源.
- 兰桑是一种具有多种生物活性的真菌化合物.
- 植物内生真菌 *Preussia* sp. 这种真菌 CPCC 401464被调查用于新代谢物生产.
研究的目的:
- 从 *Preussia* sp. 来分离和表征新的桑子衍生物.
- 在特定的化学条件下研究aflaxanthone B的结构变化.
- 评估分离化合物的抗菌潜力.
主要方法:
- 使用染色学技术将代谢物分离和净化.
- 使用1D和2D核磁共振 (NMR) 谱学和高分辨率电喷射电离化质谱学 (HRESIMS) 阐明结构.
- 通过X射线结晶学和电子循环二元化 (ECD) 计算来确定绝对配置.
主要成果:
- 阿弗拉桑B (1),一个二度四桑,从内菌中分离出来.
- 通过用二甲基硫氧化物 (DMSO) 处理aflaxanthone B来合成四种新型aflaxanthones (2-5).
- 阿弗拉桑B显示出显著的抗菌活性对抗格拉姆阳性病原性细菌,而其衍生物显示减少或没有活性.
结论:
- 阿法拉桑B的结构完整性对其强大的抗菌活性至关重要.
- 亚法兰B的化学转化导致其抗菌功效丧失.
- 这项研究突出了四桑的结构-活性关系以及它们作为抗菌剂的潜力.
更多相关视频
07:50Author Spotlight: Scalable Drug Screening Protocol for Efficient Discovery of M. abscessus Treatments
Published on: October 25, 2024
2.0K
09:09High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
Published on: November 16, 2016
8.0K
相关概念视频
01:28Antimicrobial Effectiveness
167
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
167
01:27Combined Effects of Drugs: Synergism
4.8K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
Such synergistic combinations...
4.8K
01:23Antimicrobial Proteins
5.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
5.8K