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发现FPM13的发现和冷EM结构,FPM13是一种Periplasmic金属蛋白,是弗朗西塞拉独特的

Daniel L Clemens¹, Bai-Yu Lee¹, Xiaoyu Liu^2,3

¹Department of Medicine, UCLA, Los Angeles, CA 90095, USA.

bioRxiv : the preprint server for biology

|July 14, 2025

概括

我们发现了一种新的蛋白质,FPM13,它是弗朗西塞拉细菌特有的. 这种13kDa的周等离子金属蛋白形成了一个甜甜圈形状的结构,可能在金属运输或排毒中发挥作用.

科学领域:

微生物学微生物学
结构生物学结构生物学
生物化学生物化学

背景情况:

来自弗朗西塞拉属的FTN_1118蛋白最初是在6型分泌系统 (T6SS) 功效器的净化过程中鉴定出来的.
它的功能和结构以前是未知的,需要详细的调查.

研究的目的:

确定新型弗朗西塞拉蛋白FTN_1118.18的结构和生物化学特性.
为了阐明其细胞本地化及其在弗朗西塞拉属内的潜在生物功能.

主要方法:

使用冷电子显微镜 (cryoEM) 和cryoID方法进行结构性确定.
使用了包括APEX-生物化和Triton X-114相分割在内的生物化学测定.
诱导合等离子体质谱法 (ICP-MS) 用于评估金属结合.

主要成果:

FTN_1118的结构被指定为FPM13 (弗朗西塞拉周等离子体金属蛋白,13kDa),被确定为一个独特的13kDa周等离子体蛋白.
FPM13形成了一个对称的,甜甜圈形状的18-mer复合体,其中有一个中心通道,具有β-sheet核心和α-helical shell.
生物化学分析证实FPM13是一种可溶性周等离子蛋白,可以结合铁和铜.

结论:

FPM13是一种新的,属性特定的Francisella蛋白质,具有独特的18-mer甜甜圈形结构.
FPM13是一种环等离子金属蛋白,能结合铁和铜.
虽然FPM13的删除没有影响细菌的生长,但在缺乏铁的条件下,它导致铜的敏感性增加,这表明它在金属稳态或解毒中发挥了作用.

关键词:

弗朗西塞拉弗朗西塞拉弗朗西塞拉冰冷化EMEM可以使用.金属结合蛋白质是一种金属结合蛋白质.周等离子体蛋白质的蛋白质.

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