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相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

21.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.7K
Transcription Initiation01:47

Transcription Initiation

16.8K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.8K
Leaky Scanning02:28

Leaky Scanning

5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
Initiation of Translation02:33

Initiation of Translation

34.6K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
34.6K
Real Time RT-PCR02:57

Real Time RT-PCR

59.8K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
59.8K

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相关实验视频

Updated: Sep 15, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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单细胞类型特定的替代转录启动的量化.

Shuhua Fu1,2, Parker Wilson3, Bo Zhang1,2

  • 1Department of Developmental Biology, Washington University School of Medicine, St. Louis, MO, 63110, USA.

bioRxiv : the preprint server for biology
|July 14, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了TSRdetector,这是一种生物信息工具,用于分析细胞如何使用不同的转录起始区域 (TSR). 这种方法揭示了细胞类型特定的基因调节,独立于整体表达水平.

关键词:
表达方式表达量化量化长时间阅读序列排序.一个单细胞转录组的转录组.转录启动转录启动

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

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相关实验视频

Last Updated: Sep 15, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

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Transcriptome Analysis of Single Cells
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Transcriptome Analysis of Single Cells

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 细胞利用不同的转录起始区域 (TSR) 产生各种基因异型,由转录因子调节组织特异性表达.
  • 不同TSR在各种组织和细胞类型中的选择性激活机制尚不清楚.

研究的目的:

  • 引入TSRdetector,一种新的生物信息学方法,用于在不同条件下检测TSR基因使用的显著变化.
  • 为了能够分析单细胞 (scRNA-seq) 和散装RNA测序 (bulk-RNA-seq) 数据中的差异化TSR使用情况.

主要方法:

  • TSRdetector处理RNA-seq数据以识别主导TSR并计算条件之间的差异性使用.
  • 该方法应用于人类脏snRNA-seq,小鼠B细胞分化scRNA-seq和人类ESC批量RNA-seq数据集.

主要成果:

  • 在所有测试的数据集中,TSRdetector发现了显著的TSR使用变化,与表观遗传景观变化相关.
  • 在TSR使用的变化可以修改转录异型和蛋白质产品,有时没有影响整体基因表达.
  • 观察到的TSR使用变化的很大一部分是独立于全球基因表达水平发生的,突出显示了新的调节机制.

结论:

  • TSRdetector是一个用户友好的包,用于分析从scRNA-seq和批量RNA-seq数据中差异化的TSR使用情况.
  • 该工具有助于在单细胞水平上探索替代的转录启动事件.
  • 结果揭示了独立于表达水平的独特的转录规则,影响着同型多样性.