脂肪卡德林裂变释放一个转录活性核片段来调节基因表达的目标基因表达
bioRxiv : the preprint server for biology
|July 14, 2025
概括
不典型的卡德林脂肪 (Ft) 蛋白,以前已知用于膜功能,意外地在核中发现. 这种核作用涉及调节基因表达,可能通过与Hippo通路的相互作用,补充其已知的信号功能.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 非典型的卡德林脂肪 (Ft) 对于细胞过程至关重要,如生长,平面细胞极性和跨物种的线粒体功能.
- 众所周知,它的细胞内域 (FtICD) 调节了血膜上的海马和平面细胞极性 (PCP) 途径.
研究的目的:
- 为了调查FtICD的意外核定位.
- 确定Ft及其在基因调节中的相互作用伙伴的核功能.
主要方法:
- 染色体免疫沉测序 (ChIP-seq) 用于识别Ft结合标.
- RNA测序 (RNAseq) 用于分析FT突变体中的基因表达变化.
- 共同免疫沉实验以评估蛋白质相互作用.
主要成果:
- 在培养细胞和组织中,FtICD局部化到细胞核中,需要特定的核局部化和出口信号.
- 膜结合的Ft的裂变使其能够在体内进入细胞核.
- ChIP-seq确定了许多涉及信号传递,染色体组织和发育的假定Ft基因.
- Ft在核中与Hippo通路组件Yorkie (Yki) 和Scalloped (Sd) 进行物理相互作用.
结论:
- FtICD拥有超出其已建立的膜相关作用的核功能.
- 核Ft调节Hippo通路活动并调节基因表达,可能与Yki和Sd结合.
- 这种双重定位和功能扩大了我们对Ft在细胞调节中的作用的理解.
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