相关实验视频

Updated: Jun 23, 2026

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Published on: June 5, 2012

在非洲牛载体中,对候选免疫基因表达的schistosome暴露和饮食诱导的影响

Tom Pennance, Johannie Spaan, Yijia Xiong

bioRxiv : the preprint server for biology

|July 14, 2025

概括

研究了Biomphalaria sudanica牛对Schistosoma mansoni寄生虫的免疫反应. 耐药的牛表现出更高的超氧化解突变酶1 (SOD1) 表达,饮食影响了基因表达和耐药性.

科学领域:

* 免疫学免疫学
* 寄生虫学寄生虫学
* 牛和虫的使用情况.

背景情况:

* Schistosoma mansoni是一种寄生虫虫,由淡水牛传播.
* 已知Biomphalaria glabrata对S. mansoni的免疫反应,但Biomphalaria sudanica的免疫反应却没有.
*饮食差异 (生菜与颗粒) 与牛易受S. mansoni感染有关.

研究的目的:

* 在暴露于Schistosoma mansoni之后,研究Biomphalaria sudanica中的免疫基因表达.
* 为了比较耐药和敏感的B. sudanica牛之间的免疫基因表达.
* 确定饮食对B. sudanica.免疫基因表达的影响.

主要方法:

* 逆转录定量PCR (RT-qPCR) 用于测量五个候选免疫基因的表达.
* 基因表达在暴露于S. mansoni后8,24和72小时被分析.
* 对抗性,易感性和假暴露的牛之间的表达模式进行了比较,以及牛食生菜与颗粒之间的表达模式.

主要成果:

*与敏感的牛相比,耐药的B. sudanica牛在构成性表达的超氧化物脱酶1 (SOD1) 水平更高.
*在8小时后,寄生虫暴露诱导了SOD1,生物分析素,硫蛋白1 (TEP1) 和颗粒素 (GRN) 的表达,在对生物分析素和TEP1.1敏感的牛中产生差异诱导.
*在菜养的牛中,SOD1的表达更高,与抗性增加相关.

结论:

*SOD1,生物分析素,TEP1和GRN的表达模式表明它们参与了B. sudanica的抗瘤防御.
* 增加免疫基因表达并不能保证寄生虫清除.
*饮食显著影响牛耐药性和免疫基因表达,突出了B. sudanica抗瘤反应的保存和独特方面.

更多相关视频

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

Published on: March 25, 2015

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Published on: November 17, 2020

相关概念视频

Malaria

Malaria

Malaria pathogenesis in humans reflects a delicate interplay between parasite biology and host response. Clinical illness reflects a host’s immune response to the parasite’s asexual replication cycle, which is often asymptomatic in individuals with partial immunity. From the parasite's perspective, transmission between mosquito and human with minimal host pathology is evolutionarily advantageous. Among the six Plasmodium species infecting humans, P. falciparum and P. vivax dominate in global...

Amebiasis

Amebiasis

Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...