一个prophage编码的sRNA限制了粘附性侵入性E. lytic的菌体感染. 大肠杆菌
Robert S Brzozowski1, Amelia K Schmidt1, Nicole L Pershing2
1Division of Biological Sciences, University of Montana, Missoula, MT, USA.
bioRxiv : the preprint server for biology
|July 14, 2025
概括
在大肠杆菌中,菌体编码的小RNA (svsR) 通过抑制马尔托德素运输来保护菌体感染. 这种机制增强了细菌的生存和在肠道中传播,影响了菌体的耐药性.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 病毒学 病毒学
背景情况:
- 菌体在细菌基因组中很常见,可以通过各种机制对菌体感染产生抵抗力.
- 菌体用于逃避菌体掠食的特定分子策略在诸如附着侵入性大肠杆菌 (AIEC) 等病原体中仍然在很大程度上是未知的.
研究的目的:
- 识别和描述在AIEC中介于对Lytic Coliphage的耐药性的新型prophage编码因素.
- 阐明确定因素赋予菌素耐药性的机制及其对细菌病原发生的影响.
主要方法:
- 进行比较基因组学,以识别保存的先导体元素和同类物.
- 转录分析以确定小RNA (svsR) 的基因调节.
- 营养补充实验,以评估菌体吸附动态.
- 在体内小鼠殖民模式,以评估菌体携带对菌体易感性和细菌传播的影响.
主要成果:
- 在AIEC菌株NC101中从NC-SVprophage中识别出一种小型RNA (svsR),该RNA赋予了对Lytic菌体的耐药性.
- 发现svsR抑制了马尔托德素运输基因,包括lamB,一个关键的菌体受体,从而减少了菌体吸附.
- 在体内研究表明,携带NC-SV先导体的NC101菌株经历了减少的菌体负担,并与先导体删除的菌株相比,增加了向肠外部位的传播.
结论:
- 涉及svsR的营养响应性,菌体编码机制为AIEC提供了对菌体掠食的抵抗力.
- 这种菌体抗药性策略可能有助于细菌在肠道中的持久性,并促进系统传播.
- 这些发现揭示了体元素,细菌营养代谢和体与宿主相互作用之间的新奇相互作用.
相关概念视频
01:16Viral Replication: Lysogenic Cycle
261
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
261
00:43Lysogenic Cycle of Bacteriophages
63.3K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
63.3K
01:20Viral Replication: Lytic Cycle
282
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
282
01:30Lytic Cycle of Bacteriophages
72.1K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
72.1K
01:26DNA Bacteriophages
158
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
158
01:23Stringent Response in E. coli
56
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
56