相关实验视频

Updated: Sep 15, 2025

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

同时的粒子跟踪,相位检索和点扩散函数重建

Mohamadreza Fazel^1,2,3, Reza Hoseini^1,2, Maryam Mahmoodi⁴

¹Department of Physics, Arizona State University, Tempe, AZ, USA.

bioRxiv : the preprint server for biology

|July 14, 2025

概括

本研究引入了用于3D粒子跟踪的新框架,该框架同时学习异常并重建点传播函数 (PSF). 这种方法可以提高细胞运输研究的定位精度.

科学领域:

生物物理学的生物物理.
光学显微镜的使用方法
计算生物学计算生物学

背景情况:

光生物分子的3D跟踪可以监测细胞过程.
样本诱导的波面扭曲会导致点传播函数 (PSF) 偏差,导致3D粒子跟踪中的重大定位错误.
当前的偏差校正方法,如自适应光学,需要硬件调整.

研究的目的:

开发用于同时追踪粒子,学生功能学习和直接从成像数据中重建PSF的框架.
为了克服预校准PSF和硬件依赖的偏差校正的局限性.

主要方法:

使用贝叶斯框架,在瞳孔阶段和振幅上使用连续的2D priors.
该方法从获得的3D成像数据直接重建瞳孔阶段和PSF.
使用合成和实验数据验证的框架,包括静态和扩散粒子,以及重叠PSF的多个粒子.

主要成果:

实现了同时的粒子跟踪,相检索和PSF重建.
在现实的场景中,恢复的瞳孔阶段的误差在10%以下.
恢复了10-25nm (横向) 和20-50nm (轴向) 的分衍射有限定位精度.

结论:

拟议的框架有效地纠正样本诱导的误差,而无需硬件调整.
能够实现精确的3D粒子跟踪和定位,这对于理解细胞动态至关重要.
为改善超分辨率显微镜提供了强大的数据驱动方法.

更多相关视频

Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

Three-dimensional Particle Tracking Velocimetry for Turbulence Applications: Case of a Jet Flow

Published on: February 27, 2016

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...