组织样本制备方法对髓敏感量的MR成像技术的影响
Amaya Murguia1, Scott D Swanson2,3, Ulrich Scheven4
1Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, USA.
bioRxiv : the preprint server for biology
|July 14, 2025
概括
结是一种可行的替代品,用于肌敏感定量MRI (qMRI) 的组织固定. 虽然化后的qMRI值可能会略有增加,但组织学证实组织完整性得到维持,支持结用于qMRI分析.
科学领域:
- 神经成像是一种神经成像.
- 历史学 历史学 历史学
- 生物医学工程 生物医学工程
背景情况:
- 定量MRI (qMRI) 参数验证通常使用固定的ex vivo组织.
- 冷和解对髓敏感的qMRI参数及其组织学相关性的影响需要进一步研究.
研究的目的:
- 评估冷和解对髓敏感的qMRI参数的影响.
- 为了将qMRI结果与新鲜,解和固定羊脑组织的组织学染色 (Luxol快速蓝色) 进行比较.
主要方法:
- 进行了髓水成像,非共振射频和磁化转移 (MT) 和选择性反转恢复MTMRI.
- 14个羊脑组织样本在新鲜,解和固定状态下进行了分析.
- qMRI衍生的髓替代物测量与Luxol快速蓝色 (LFB) 染色进行了比较.
主要成果:
- qMRI参数在所有组织状态中与LFB组织学有很好的相关性.
- 与新鲜组织相比,解和固定组织在大多数qMRI参数中显示了适度增加 (3-32%).
- 组织学证实,解后的样本在冷后保持了组织完整性.
结论:
- 结是一种适合的替代方法,用于对髓敏感的qMRI分析.
- 结和解可能会导致qMRI参数值的差异变化,这取决于区域髓含量.
- 通过冷来保持组织完整性,使其成为QMRI研究的实用方法.
相关概念视频
01:24Magnetic Resonance Imaging
7.2K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
7.2K
01:20Preparation of Samples for Electron Microscopy
5.9K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.9K