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Updated: Sep 15, 2025

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
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使用自组装标签对蛋白质滴状形成能力的细胞内评估

Takayuki Miki1,2, Masahiro Hashimoto1, Masatoshi Shimizu2

  • 1School of Life Science and Technology, Tokyo Institute of Technology 4259 Nagatsuta-cho, Midori-ku Yokohama Kanagawa 226-8501 Japan.

Chemical science
|July 14, 2025
PubMed
概括

研究人员开发了一种新的测试方法来研究细胞内蛋白质滴滴的形成. 这种方法使用标签来诱导蛋白质的自我组装,揭示了对细胞聚合和反应的新见解.

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Tagging and Fusion Proteins01:24

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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