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使用可激活光开关进行单一可见光STORM成像

Fanghui Li¹, Mengqi Li¹, Yiqi Shi¹

¹Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Shanghai Key Laboratory of Functional Materials Chemistry, Institute of Fine Chemicals, East China University of Science & Technology Shanghai 200237 China whzhu@ecust.edu.cn zhwj@ecust.edu.cn.

Chemical science

|July 14, 2025

概括

研究人员开发了用于超分辨率显微镜的新型光开关. 这些开关通过可见光激活并检测细胞内部的生物分子,简化了静态光学重建显微镜 (STORM) 成像.

科学领域:

生物医学成像技术生物医学成像技术
纳米技术纳米技术
有机化学有机化学

背景情况:

随机光学重建显微镜 (STORM) 实现了纳米级分辨率,但需要先进的,依赖紫外线光的光开关.
目前的光开关对生物分子缺乏特异性,需要复杂的双光束设置.

研究的目的:

为STORM成像设计由单一可见光源激活的光开关.
为了创建可被细胞内生物分子激活的光开关,用于有针对性的超高分辨率成像.

主要方法:

将分子内质子转移 (IPT) 单元纳入光色二乙烯,以创建可见光驱动的光开关.
具有生物分子识别动机的IPT单元的功能化,用于检测特定的细胞内生物分子 (例如GSH,β-Gal).

主要成果:

开发了全可见光驱动的光开关,具有高光响应效率,亮度和光对比度.
使用单一可见光 (488 nm) 源的STORM成像成功证明.
创建了能够感知超分辨率的细胞内生物分子表达水平的光开关.

结论:

新的光开关通过使用单一可见光源来简化STORM成像.
生物分子可激活的光开关扩大了超分辨率显微镜的应用,用于精确的细胞内映射.
这一进步为纳米尺度生物成像提供了更简单,更通用的方法.

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