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一个来自异黄的AIE活性探针用于过氧酸盐检测和生物成像应用

Raguraman Lalitha¹, Vishnu Halnor Vivek¹, Yueh-Hsun Lu²

¹Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology Tiruchirappalli - 620 015 India velmathis@nitt.edu.

|July 14, 2025

概括

一个基于异子的新型探针 (IHP) 通过聚合诱导排放 (AIE) 检测过氧酸盐 (ONOO-). 这种AIE探测器在生物系统和活细胞中显示出高灵敏度和选择性的ONOO检测.

科学领域:

化学传感器化学传感器
材料科学材料科学材料科学
生物物理化学生物物理化学

背景情况:

聚合诱导排放 (AIE) 化合物由于受到限制的分子内旋转,因此具有独特的光物理特性.
过氧化 (ONOO-) 是一种反应性物种,涉及各种生理和病理过程.
开发用于ONOO检测的选择性和敏感的探针对于生物研究至关重要.

研究的目的:

设计和合成一种基于异子的探针 (IHP) 用于过氧酸盐 (ONOO-) 检测.
研究IHP的聚合行为和光物理性质.
为了评估探针的性能选择性和敏感的ONOO-检测在体外和活细胞.

主要方法:

使用C=N反应组合成基于异子的探针 (IHP).
在不同的溶剂系统 (DMSO:PBS和DMSO) 中进行光物理研究,以分析聚合行为.
光谱法用于监测探头对过氧酸盐的反应.
细胞成像实验可视化活细胞中的过氧酸盐.

主要成果:

探测器IHP表现出聚合诱导发射 (AIE) 特性,具有红色发射.
IHP显示DMSO:PBS中的波长依赖的光变化,表明J-聚合.
探测器通过C=N键的氧化裂变选择性地与过氧酸盐反应,导致排放的转移.
获得了高灵敏度 (检测极限1.1μM) 和快速响应 (<200秒).
证明了活细胞内外源性和内源性过氧酸盐的有效成像.

结论:

开发的基于异氧的探头 (IHP) 有效地利用AIE进行敏感和选择性的过氧酸盐检测.
探测器能够在活细胞中对过氧酸盐进行成像,这为生物和生物医学应用开辟了道路.
这项工作突出了AIE探测器在化学传感和生物成像方面的潜力.

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