TRPC4调节序列的分子进化支持哺乳动物热调节适应中的作用
1Fort Collins Science Center, U.S. Geological Survey, Fort Collins, CO, United States of America.
PeerJ
|July 14, 2025
概括
对Trpc4的进化,Trpc4是一种关键的基因,对非发热生成 (NST),显示与哺乳动物的温度调节策略一起适应. 它的速度随着棕色脂肪组织 (BAT) 的增加而增加,但在大多数后代中变得更加受限制,在蝙蝠中显著的例外.
科学领域:
- 进化生物学是进化的生物学.
- 分子生物学分子生物学
- 身体生理学 身体生理学
背景情况:
- 规范性短暂受体潜力 (Trpc) 蛋白质形成对信号转导至关重要的跨膜通道.
- 在小鼠中,Trpc4对于非发热生成 (NST) 和棕色脂肪组织 (BAT) 功能至关重要.
- Trpc4表现出蝙蝠的多样化选择,这表明在监管区域的适应.
研究的目的:
- 调查Trpc4序列多样化是否与哺乳动物温度调节策略的演变有关.
- 为了确定不同哺乳动物群中的Trpc4的进化压力及其发热能力.
- 评估Trpc4的进化轨迹与BAT存在和损失有关.
主要方法:
- 估计了四足动物外组和eutherian序列中Trpc4的非同义和同义替代 (ω) 的比率.
- 应用分支站点模型和RELAX程序来检测积极选择和进化约束的变化.
- 分析了Trpc4和相互作用基因 (Trpc1,Trpc5) 的进化速率,与哺乳动物类别的异热度水平相关.
主要成果:
- 随着BAT的出现,Trpc4的进化速率在祖先的eutheria中增加了,但在后代类中变得更加受限制.
- 在二次失去BAT的基层中观察到对Trpc4的减少约束.
- 蝙蝠表现出Trpc4和相互作用基因的进化率增加,这表明限制放松和选择多样化,而动物表现出低率.
结论:
- TRPC4的C终端区域是通过与NST热调节相关的选择在eutherian多样化过程中形成的.
- Trpc4的进化反映了对哺乳动物不同温度调节需求的适应性反应.
- 增加蝙蝠Trpc4多样化的特定驱动因素需要进一步调查.
关键词:
适应 适应 适应棕色脂肪组织的脂肪组织.进化速率是指进化的速度.哺乳动物 哺乳动物分子进化的分子进化.没有的热生成.积极的选择选择是积极的选择.热调节 热调节 热调节暂时的受体潜在通道是暂时的受体.更多相关视频
12:09Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
Published on: December 31, 2013
10.2K
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
9.6K
相关概念视频
01:26Thermoregulation
1.3K
The human body has a sophisticated thermoregulation system that employs negative feedback mechanisms to maintain an optimal core temperature. When the core temperature drops, peripheral and central thermoreceptors send signals to the hypothalamus, activating the heat-promoting center. This center triggers several responses aimed at increasing the core temperature. First, vasoconstriction reduces the flow of warm blood from internal organs to the skin so that the heat is not lost from the skin,...
1.3K
01:43Thermosensation
31.8K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
31.8K
02:02Cis-regulatory Sequences
10.1K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
10.1K
02:23Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
01:16Transduction
120
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
120
02:05Gene Evolution - Fast or Slow?
7.4K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.4K