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开发和验证UHPLC-MS/MS方法用于血中检测西普罗:在临床药理动力学研究中的应用

Jiaxi Zhu^1,2, Ying Cao², Xingan Zhang²

¹The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, 510515, People's Republic of China.

Drug design, development and therapy

|July 14, 2025

概括

一种新的超高性能液态色谱-并联质谱法 (UHPLC-MS/MS) 方法准确地量化了人体血中的西普罗. 这种经过验证的方法对于手术患者的药理动力学研究至关重要.

科学领域:

分析化学分析化学
药理动力学药理动力学
质谱测量质量谱测量

背景情况:

西普罗是一种麻醉剂,用于全身麻醉.
准确量化血中的西普罗对于药理动力学研究至关重要.
现有的分析方法可能对临床应用来说不够敏感或特定.

研究的目的:

开发和验证一种超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 方法.
在人体血样本中量化西普罗.
为了使在接受手术的患者中进行药理动力学研究.

主要方法:

样品的制备过程涉及以甲醇为基础的蛋白质沉,其内部标准是醇-d6.
在C18柱上进行了染色分离,使用酸和甲醇作为移动相.
检测利用了负离子模式中的电喷射电离 (ESI) 与多重反应监测 (MRM).

主要成果:

该方法在广泛的度范围 (5-5000 ng·mL−1) 上显示出出色的线性.
实现了高精度和准确性,在可接受的范围内,在批量内和批量间的变化.
提取回收率很高 (87.24%97.77%),矩阵效应最小.

结论:

开发的UHPLC-MS/MS方法简单,快速,准确,高度具体.
这种经过验证的方法适用于在临床环境中确定西普罗的血度.
该方法为未来的西普罗的药理动力学研究提供了可靠的工具.

关键词:

在UHPLC-MS/MS中使用.酸 (Ciprofol) 是一种酸.药物动力学药物动力学

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