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相关概念视频

Immunoprecipitation01:20

Immunoprecipitation

5.9K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.9K

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相关实验视频

Updated: Sep 15, 2025

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

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使用蛋白质组学数据成功进行免疫细胞解的考虑因素和软件.

Måns Zamore1, Sergio Mosquim Junior1, Sebastian L Andree1

  • 1Department of Immunotechnology, Lund University, SE-22363 Lund, Sweden.

Journal of proteome research
|July 14, 2025
PubMed
概括
此摘要是机器生成的。

蛋白质组学数据现在可以在批量样本中估计免疫细胞组成. 这项研究验证了计算方法,并引入了使用蛋白质组学进行细胞解的R包,在模拟中实现了高精度.

关键词:
这是LC-MS/MS.细胞类型的解解.免疫细胞 免疫细胞免疫透 免疫透是什么?蛋白质组学 蛋白质组学

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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相关实验视频

Last Updated: Sep 15, 2025

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 计算生物学 计算生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 大量转录是细胞类型解卷的常见现象.
  • 蛋白质组学数据在细胞解体过程中未得到充分利用.
  • 估计细胞组成可以提供生物学见解.

研究的目的:

  • 用批量蛋白质组学数据评估免疫细胞解的计算方法.
  • 评估预处理和软件对解卷精度的影响.
  • 介绍一种基于蛋白质组学的细胞解的工具.

主要方法:

  • 利用定义的免疫细胞种群和模拟混合物.
  • 评估各种预处理技术和软件工具.
  • 开发并验证了一个名为proteoDeconv.的R包.

主要成果:

  • 使用蛋白质组学数据证明了细胞类型解的可行性.
  • 在模拟混合物中的估计比例中达到0.9以上的皮尔森相关性.
  • 确定了缺失值赋值和参考矩阵生成的最佳参数.

结论:

  • 蛋白质组学数据是细胞类型解的可行替代方案.
  • 蛋白质Deconv R包简化了蛋白质学数据预处理以进行解卷.
  • 这种方法增强了对生物样本中细胞组成的分析.