在鱼 (Procambarus clarkii) 中使用弗洛芬尼科尔的种群药理动力学,基于稀疏采样方法和非线性混合效应模型
Ning Xu1, Juan Tian1, Binbin Gong2
1Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.
Journal of veterinary pharmacology and therapeutics
|July 14, 2025
概括
在鱼中,使用非线性混合效应模型确定了florfenicol (FLO) 的种群药动力学. 这项研究提供了一种简洁的方法,用于水产养殖中的药理动力学分析,这对于类动物的药物开发至关重要.
科学领域:
- 药理学 药理学是指药理学的学科.
- 水产养殖科学 水产养殖科学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 佛罗芬尼科尔 (FLO) 是一种用于水产养殖的抗生素.
- 了解其在像鱼 (Procambarus clarkii) 等甲动物中的药理动力学 (PK) 是有效的治疗方案的关键.
- 种群药动力学 (PPK) 模型为分析不同动物种群中药物处置提供了强大的框架.
研究的目的:
- 为了确定口服后鱼 (Procambarus clarkii) 中的花尼科尔 (FLO) 的种群药动力学 (PPK).
- 调查身体重量和性别等共变量对FLO药理动学的影响.
- 开发和验证PK在甲动物物种研究的简洁方法.
主要方法:
- 一次口服剂量为15 mg/kg FLO,在25°C的温度下给了32只鱼.
- 采用稀疏的血液采样,每个动物在三个时间点取样.
- 具有紫外线检测的高性能液态色谱 (HPLC) 用于样本量化.
- 用于PPK分析,使用非线性混合效应模型 (NLME),结合附加误差和共变模型.
主要成果:
- NLME模型成功地描述了鱼中的FLO药理动力学.
- 发现体重和性别对FLO PK参数没有显著影响.
- 估计的关键PK参数包括1.93/h的吸收速率常数,11.16 L/kg的明显分布体积和2.35 L/h/kg的总系统清除量.
- 消除半衰期被确定为3.47小时.
结论:
- 开发的PPK模型为鱼中的花尼科尔排放提供了宝贵的见解.
- 这项研究展示了在水产养殖中适用于甲类动物进行PK研究的简化方法.
- 这种方法支持花尼科尔的合理使用,并有助于在水产养殖中制定PK导向的剂量策略.
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