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Updated: Jun 20, 2026

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
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工程 CCL2 化学基因单体的结构-动力学-功能关系的NMR阐明

Deepak Kumar Tripathi1, Khushboo Gulati1, Siddhartha Das Pramanik1

  • 1Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, India.

Magnetic resonance in chemistry : MRC
|July 14, 2025
PubMed
概括

设计的单体单细胞化学吸引蛋白-1 (CCL2-M) 呈现出与其二维形式 (CCL2-WT) 不同的结构动态和稳定性. 尽管稳定性较低,但CCL2-M有效诱导单细胞迁移,类似于CCL2-WT.

关键词:
化学物质 (chemokines) 是一种化学物质.血红细胞的招募.一个单细胞的化学吸引蛋白质.核磁共振光谱学 核磁共振光谱学蛋白质工程工程 蛋白质工程

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¹H NMR: Complex Splitting01:13

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A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

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¹H NMR: Long-Range Coupling01:27

¹H NMR: Long-Range Coupling

The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
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