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对于人类核心眼皮表面微生物群的特征
Sophia C Morandi1,2, Anne-Christine Uldry2, Nina Eldridge1
1Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Investigative ophthalmology & visual science
|July 14, 2025
概括
这项研究使用全元基因组测序定义了健康的核心眼表面微生物群 (COSM). 诸如Cutibacterium acnes和Staphylococcus epidermidis之类的关键物种构成了COSM,对于了解眼睛表面健康和疾病至关重要.
科学领域:
- 微生物学 微生物学
- 眼科医生 眼科 眼科
- 转基因组学是指转基因组学.
背景情况:
- 眼表面微生物组 (OSM) 在眼表面 (OS) 的健康和疾病中起作用.
- 目前的OSM数据不一致,需要标准化描述.
- 了解健康的OSM对于识别与疾病相关的变化至关重要.
研究的目的:
- 使用全元基因组猎枪测序来描述健康的核心眼表面微生物群 (COSM).
- 为所有分类学王国提供OSM的物种级别解析.
- 建立未来研究OSM和眼睛表面疾病的基准.
主要方法:
- 在3个时间点从27名健康个体收集了结膜,眼边缘和液样本.
- 使用全元基因组猎枪测序对OSM进行了测序,并使用Kraken2和HUMAnN3.3进行了分析.
- 通过使用nLC-MS/MS和基因本体学来表征蛋白质组 (TP) 验证了这些发现.
主要成果:
- 确定了Cutibacterium acnes,Malassezia restrita和Staphylococcus epidermidis作为COSM的核心物种.
- 鉴定出Corynebacterium segmentosum是核心盖微生物组 (LM) 的一部分.
- 观察到OSM和TP的显著个体间变化,超过了个体内差异.
结论:
- 定义的COSM作为眼睛表面微生物组研究的基础参考.
- 这种表征可能会为眼睛表面疾病的未来预防和治疗策略提供信息.
- 个体特定的微生物群签名是眼睛表面环境的关键特征.
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