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外基因介导的circRNA Hsa_Circ_0113050通过与EIF4A3相互作用,增强结肠直肠癌细胞恶性

Yuan Tian¹, Chen He²

¹Department of Pain Treatment/Hepatobiliary Surgery/Vascular Surgery/Gastrointestinal Surgery, Affiliated Hospital of Jianghan University (The Sixth Hospital of Wuhan), Wuhan, China.

DNA and cell biology

|July 14, 2025

概括

携带圆形RNA hsa_circ_0113050的外基因组促进结直肠癌 (CRC) 的生长和扩散. 这种循环RNA与EIF4A3相互作用,为CRC治疗提供了一个潜在的新点.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
生物化学生物化学

背景情况:

循环RNAs (circRNAs) 与癌症的发展有关.
外基因组促进细胞间通信,并可以运输功能分子,如circRNAs.
在结直肠癌 (CRC) 中外体介导circRNAs的特定作用需要进一步阐明.

研究的目的:

为了研究外体介导circRNA hsa_circ_0113050在结直肠癌 (CRC) 中的作用.
在CRC中探索hsa_circ_0113050和真核细胞翻译启动因子4A3 (EIF4A3) 之间的相互作用.
评估hsa_circ_0113050作为CRC的治疗点的潜力.

主要方法:

使用超离心,传输电子显微镜和纳米粒子追踪分析,对CRC衍生的外体进行隔离和表征.
通过生物信息分析和qRT-PCR对CRC组织和外体中的hsa_circ_0113050表达的量化.
功能性试验 (基于细胞和体内) 来评估外体和hsa_circ_0113050对CRC细胞恶性病变的影响.
通过RNA免疫沉,西式涂抹和qRT-PCR进行hsa_circ_0113050-EIF4A3相互作用的分析.

主要成果:

来自CRC的外体 (102-104nm) 显著增强了CRC细胞的增殖,迁移和入侵.
hsa_circ_0113050在CRC组织和外体细胞中被上调.
在外体内静止hsa_circ_0113050扭转了亲恶性瘤效应.
发现EIF4A3与hsa_circ_0113050 (EIF3I) 的线性前体结合,从而促进hsa_circ_0113050在CRC细胞中的表达.

结论:

外基因介导的hsa_circ_0113050通过与EIF4A3.3的相互作用促进结直肠癌细胞恶性病变.
这项研究阐明了CRC中circRNA调节的新型机制.
外体 hsa_circ_0113050 是结直肠癌的一个有前途的治疗点.

关键词:

欧洲投资基金4A3A3 环RNA 环RNA 是一个环RNA.结肠直肠癌是什么意思外基因组是外基因组的组成部分.hsa_circ_011305050 在线查看

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