相关实验视频

Updated: Sep 15, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

Published on: March 14, 2013

提高初级新生儿查先天性上腺增生与LC-MS/MS/MS的效率

Alexander Gaudl¹, Laura Lehmicke¹, Ronald Biemann¹

¹Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, Leipzig University Medical Center, Leipzig, Germany.

Clinica chimica acta; international journal of clinical chemistry

|July 14, 2025

概括

与传统的免疫检测方法相比,一种新的快速LC-MS3检测显著改善了先天性上腺增生 (CAH) 新生儿查,减少了假阳性并提高了诊断效率.

科学领域:

生物化学生物化学
质谱测量质量谱测量
临床诊断临床诊断临床诊断

背景情况:

使用免疫试验 (IA) 对新生儿先天性上腺增生 (CAH) 的查 (NBS) 面临分析挑战.
现有的双重质谱法对于高通量选来说太慢了.

研究的目的:

为了评估CAH NBS的快速1.5分钟LC-MS3测定.
为了提高诊断准确度,确定成熟度依赖的切断值.
为了比较LC-MS3的诊断效率与IA用于CAH查.

主要方法:

使用在线SPE-LC-MS3在1730个残留干血样本中量化17-基 (17-OHP).
在MS3分析中使用了两阶段的碎片化 (m/z 329/285/123).
根据怀孕年龄 (GA) 和出生体重 (BW) 计算了成熟度依赖的切断值.

主要成果:

在LC-MS3测定显示了高精度 (10-13%) 和准确性 (99-101%).
取决于到期的切割值将虚假阳性从731个减少到15个,同时正确识别所有CAH病例.
对CAH查的积极预测值从1%增加到32%.

结论:

结合LC-MS3和依赖到期的切断值,可显著提高CAH NBS诊断效率.
这种方法比传统的IA方法具有重大优势.
在新生儿查中,MS3技术具有很强的临床应用潜力.

关键词:

年龄和体重调整后的截止值.临床质谱学临床质谱学遗传性上腺过度增生诊断效率的提高提高诊断效率的提高离子陷定量测量离子陷定量测量新生儿查新生儿查

更多相关视频

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...