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Updated: Sep 15, 2025

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
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使用时间动力学的定量相位成像预测了造血干细胞多样性的预测.

Takao Yogo1, Yuichiro Iwamoto2, Hans Jiro Becker3

  • 1Division of Cell Regulation, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. takayogo0430@g.ecc.u-tokyo.ac.jp.

Nature communications
|July 14, 2025
PubMed
概括

这项研究引入了一种新的方法,用于使用活细胞成像和机器学习来预测造血干细胞 (HSC) 多样性. 它通过分析随时间推移的细胞行为来揭示以前隐藏的HSC变异,改善干细胞治疗预测.

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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