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Updated: Sep 15, 2025

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
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Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

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通过计算机辅助结构指导设计,调节P450BM3单氧化酶对N异环的N氧化选择性

Liu Yang1,2, Zhongji Pu3, Jianping Wu1,2

  • 1Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.

Nature communications
|July 14, 2025
PubMed
概括

这项研究使用P450BM3酶工程来增强N-异环的N-氧化. 优化突变物实现高选择性和转化率,以实现高效的氧化合成.

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