多基因 luciferin 生物合成途径的表达在植物中作为伪多基因的表达
David Samson1, Natalie S Thompson1, Vijay R Sheri1,2
1Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.
Scientific reports
|July 14, 2025
概括
这项研究在植物中体内证明了露西芬的生物合成,使其在没有外部基质的情况下能够产生生物发光. 然而,基质不稳定性和植物物种差异对报告员应用提出了挑战.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 植物科学 植物科学
背景情况:
- 生物发光依赖于一种素/酶系统.
- 在体内生产露西费林为外源基质添加提供了替代品.
- 关键的酶包括酸乙烯转移酶 (NpgA) 和素生物合成途径中的酶.
研究的目的:
- 在植物中使用多基因基因构造来证明路西费林生物合成.
- 评估生物发光作为植物中记者系统的有效性.
- 调查与露西费林稳定性和特定植物物种火效应相关的挑战.
主要方法:
- 构建了一种表达 luciferin 生物合成途径基因的多基斯特龙基因,使用 intein-F2A .
- 在Nicotiana benthamiana和西红 (Solanum lycopersicum) 中表达的基因用于生物发光测试.
- 使用Agrobacterium表达光酶作为生物传感器用于光素和植物组织火.
主要成果:
- 在N. benthamiana中与共同表达的基因实现了生物发光,但在西红中没有.
- 在N. benthamiana中,NpgA表达增强了生物发光,当它从LBS多晶体中分离出来时.
- 观察到显著的植物物种依赖的火,特别是在番茄中,阻碍了露西芬的检测.
- 路西弗林的不稳定性被确定为一个限制因素.
结论:
- 在植物中, luciferin 生物合成是可行的,但由于基质的不稳定性和植物特异性火而受到挑战.
- 的 luciferase 作为一个敏感的生物传感器用于 luciferin 和组织火.
- 对于强大的转基因记者应用程序,需要在路径工程方面进一步改进.
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