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快速,经济高效和灵活的DNA测序通过卷对卷流体学

Yanzhe Qin^1,2, Stephan A Koehler³, Yunyan Ling⁴

¹BGI Research, Shenzhen, China. yanzheqin@126.com.

|July 14, 2025

概括

下一代测序 (NGS) 的性能通过基于Couette流的滚滚流体 (r2r-fl) 来提高. 这种具有成本效益的方法显著减少了用于诊断和基因分析的试剂使用和测序时间.

科学领域:

生物技术是生物技术.
基因组学就是基因组学.
流体动力学流体动力学

背景情况:

下一代测序 (NGS) 已经彻底改变了生物研究和临床诊断.
在NGS的性能改进由于流动细胞中Poiseuille流动力学的限制而停滞不前.

研究的目的:

通过实施Couette流来克服NGS性能方面的局限性.
引入一个具有成本效益和可扩展的NGS方法,使用卷对卷流体 (r2r-fl).

主要方法:

通过卷对卷流体学 (r2r-fl) 开发了NGS,这是平面Couette流的实际实现.
为了兼容性和成本效益,采用了灵活的生物芯片大小.

主要成果:

实现了高达85倍的试剂消耗降低 (每千兆基对0.16美元).
将冲洗时间缩短到2秒以下,并将周转时间从几天减少到12小时以下.
保持高精度:单核酸多态性 (SNP) 的精度>99.9%,大肠杆菌的映射率为99.9%,SARS-CoV-2的错误最小.

结论:

r2r-fl通过克服流体动力学限制,在NGS技术方面取得了重大进展.
该方法的成本和时间降低使得在病原体检测,癌症诊断和遗传疾病分析方面能够快速,可扩展的应用.

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