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在小麦基因转换中TaWOX14的功能

Dan Wang¹, Yajie Guo¹, Mengtian Liu¹

¹College of Agronomy and the State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, Henan, China.

Plant cell reports

|July 14, 2025

概括

小麦TaWOX14的过度表达增强了基因转换和基因组编辑. 鉴定出TaPLD是一种潜在的单 haploid 诱导基因,加速了小麦育种的进步.

科学领域:

植物科学植物科学
遗传学是一个遗传学.
农业生物技术农业生物技术

背景情况:

基因转换和基因组编辑对于作物改进至关重要,但往往受到扩展性再生效率的限制.
众所周知,与WUSCHEL相关的homeobox (WOX) 基因家族,特别是WUS类,对于植物体质胚胎发生至关重要.
提高再生效率是加速作物育种计划的关键.

研究的目的:

研究WUS类基因在小麦转化中的作用.
使用TaWOX14提高小麦的基因转换和基因组编辑效率.
探索TaPLD作为在小麦中诱导平分体的候选基因.

主要方法:

在小麦转化过程中对WUS类基因进行系统分析.
在多个基因型的小麦不成熟胚胎中过度表达TaWOX14.
结合CRISPR-Cas9系统与TaWOX14进行基因组编辑.
编辑Zea mays PHOSPHOLIPASE D3 (ZmPLD3) 的小麦同类物,以确定TaPLD在平分体诱导中的作用.

主要成果:

过度表达TaWOX14显著提高了小麦基因型Fielder,Kenong199,Zhengmai7698和Yangmai13中的遗传转换效率.
在使用CRISPR-Cas9系统与TaWOX14结合使用时,在小麦品种Fielder中观察到增强的基因组编辑效率.
鉴定出了一种TaPLD编辑的植物,其ploidy水平在 haploid 和 hexaploid 之间是中间的,这表明它有可能诱导 haploid.

结论:

过度表达TaWOX14是一种可行的策略,可以显著提高小麦遗传转换和基因组编辑效率.
塔普尔德基因 (TaPLD) 成为促进小麦中平分体诱导的有希望的候选基因.
这些发现共同有助于通过增强的转化和新的单 haploid 诱导方法加速小麦育种.

关键词:

我们的 TaWOX14 在 ZmPLD3D3 中.基因转换是一种基因转换.植物再生植物再生小麦小麦小麦的小麦.

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