在液性HSV-1和HSV-2感染期间,人类前皮纤维细胞中的血清型特异性宿主蛋白质重塑
Xiaohong Pan1, Jiaxin Xie1, Zhidan Zhang1,2
1Yunnan Provincial Key Laboratory of Vector-Borne Diseases Control and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming, China.
Virology journal
|July 14, 2025
概括
这项研究揭示了简单疹病毒1型 (HSV-1) 和2型 (HSV-2) 蛋白质之间的差异,影响宿主细胞. 了解这些简单疹病毒的差异是解释它们独特的疾病影响的关键.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 简单疹病毒1型 (HSV-1) 和2型 (HSV-2) 引起不同的疾病,但它们不同的致病性分子原因尚未完全理解.
- 在蛋白质水平上研究宿主-病原体相互作用对于破译血清型特异性毒性至关重要.
研究的目的:
- 进行人类前皮纤维细胞 (HFF-1) 感染HSV-1和HSV-2的细胞的比较蛋白质组分析.
- 确定差异表达蛋白 (DEPs) 并阐明背后的HSV-1和HSV-2独特致病性背后的分子机制.
主要方法:
- 采用数据独立采集质谱法 (DIA-MS) 进行定量蛋白质组分析.
- 在感染HSV-1和HSV-2的炎症感染期间分析了受感染的人类前皮纤维细胞 (HFF-1) 细胞.
主要成果:
- 在感染HSV-1的细胞中发现了280个DEP,在感染HSV-2的细胞中发现了219个DEP.
- HSV-1上调抗病毒蛋白 (ISG20,IRF7) 和脂质代谢,同时降低化学激素信号的调节.
- 在HSV-2上调节了炎症蛋白 (IGHV3-9,SERPINA1),增强了NF-κB信号传递,改变了糖代谢.
结论:
- HSV-1抑制了免疫逃避的炎症和抗原呈现途径.
- HSV-2 诱导更强的促炎反应和代谢重编程.
- 这些蛋白质组差异为HSV-1和HSV-2的血清型特异性病原发生提供了洞察力.
相关概念视频
01:28Herpes
Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
01:23Genital Herpes
Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...