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Updated: Sep 15, 2025

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

通过MCT1介导的乳酸运送到线粒体,控制巨细胞的极化,通过影响β细胞来调节葡萄糖平衡

Lingling Chen¹, Yijun Lin², Xinyu Zhu³

¹CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|July 15, 2025

概括

单碳酸盐输送体 (MCTs) 调节巨细胞的两极分化. MCT1将乳酸导向线粒体,这对葡萄糖平衡至关重要,而MCT4则促进乳酸输出.

科学领域:

免疫学免疫学免疫学
代谢途径代谢途径
细胞生物学细胞生物学

背景情况:

乳酸燃料的三碳酸循环和影响巨细胞的功能通过基因素乳化.
单碳酸盐输送体 (MCT) 在巨细胞极化中的特定作用尚未完全理解.

研究的目的:

研究MCT1和MCT4在巨细胞极化中的不同作用.
阐明 MCT 调节巨细胞功能和葡萄糖平衡的机制.

主要方法:

在M1和M2巨细胞极化过程中分析MCT1和MCT4表达.
细胞和动物模型评估MCT1/MCT4操纵对巨细胞功能和葡萄糖代谢的影响.
研究参与乳酸介导胰岛素分泌的信号通路.

主要成果:

随着M1极化,MCT4表达增加,局部化到血膜,促进乳酸排泄.
随着M2极化,MCT1表达增加,局部化到线粒体,促进细胞内乳酸流.
阻断MCT1会使M1极化恶化,损害线粒体功能,加剧葡萄糖不耐受,增加小鼠岛屿细胞死亡.
乳酸盐通过GPR81-cAMP-PKA通路抑制胰岛素分泌.

结论:

通过MCT1介导的线粒体乳酸运输对于调节巨细胞两极分化至关重要.
巨细胞和胰腺β细胞功能互动以维持葡萄糖平衡,其中MCT1起着关键作用.

关键词:

在MCT1中,MCT1是MCT1.葡萄糖的恒常性是葡萄糖的恒常性.乳酸穿机乳酸穿机巨细胞是一个巨细胞.线粒体中的线粒体.胰腺小岛是一个胰腺小岛.

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