Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Reporter Genes02:11

Reporter Genes

11.9K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dual-modular-nanobody CAR-T cell technical platform against the solid tumor microenvironment.

Journal of hematology & oncology·2026
Same author

Author Correction: Quantification and transcriptome profiling reveal abundant, dynamic and translatable dephospho-CoA-capped RNAs.

Nature biotechnology·2026
Same author

Association between hemoglobin glycation index and all-cause mortality in patients with non-ST-segment elevation myocardial infarction undergoing percutaneous coronary intervention.

Frontiers in endocrinology·2026
Same author

Graphene field-effect transistor microarray with simple preparation method, good stability, and high sensitivity for bioassay.

Biosensors & bioelectronics·2026
Same author

Enhancement of the Anti-Hepatoma Activity of Acacetin Through Salt Formation of Sodium Acacetin.

Chemistry & biodiversity·2026
Same author

Enzymatic Redox Gating Directs Oxidative Divergence in Acyclic Peroxides Biosynthesis.

Angewandte Chemie (International ed. in English)·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
查看所有相关文章

相关实验视频

Updated: Sep 15, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.9K

基于RNA的光传感器与RhoBAST

Haozhi Song1,2,3, Zhayila Reheman4, Ying Fang4

  • 1Interdisciplinary Science Center, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.

Analytical chemistry
|July 15, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的红色光RNA生物传感器,用于成像多个细胞目标. 这种明亮,稳定的传感器克服了以前的RNA传感器的局限性,使活细胞中的亚细胞成像成为可能.

更多相关视频

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.6K
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
08:11

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

1.9K

相关实验视频

Last Updated: Sep 15, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.9K
Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
12:23

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

14.6K
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
08:11

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

1.9K

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 细胞生物学 细胞生物学

背景情况:

  • 基于RNA的生物传感器用于细胞目标成像.
  • 现有的RNA传感器的亮度低,光稳定性不佳,动力学缓慢,背景噪音绿色.
  • 当前的传感器往往无法在亚细胞区内检测多个目标或图像.

研究的目的:

  • 开发一种基于RNA的新型传感器,以提高细胞成像的性能.
  • 为了使多个目标同时进行检测和亚细胞成像.
  • 克服现有的RNA生物传感器的局限性.

主要方法:

  • 开发一种基于RNA的新型传感器,使用RhoBAST化RNA吸收剂.
  • 传感器用于检测活细胞中的小分子和离子 (Ca2+).
  • 创建一个直角传感器,同时对多个小分子进行亚细胞成像.

主要成果:

  • 基于RhoBAST的传感器表现出高红色光强度,优越的光稳定性和快速激活动力学.
  • 开发的传感器在关键性能指标上优于以前的光传感器.
  • 通过使用直角传感器设计,实现了多个小分子的同时亚细胞成像.

结论:

  • 一个新的,高亮度,稳定,快速响应的红色光RNA传感器已经开发出来.
  • 这种传感器技术解决了现有的基于RNA的生物传感器的关键局限性.
  • 该传感器可以在活哺乳动物细胞内的亚细胞多目标成像中实现先进的应用.