脊柱刚性编码生物分子凝结体中的通用粘弹性特征
bioRxiv : the preprint server for biology
|July 15, 2025
概括
将链条刚性引入到内在无序蛋白质模型中,可以准确地预测生物分子凝结物的粘性弹性. 这一进步揭示了序列如何决定材料特性和弹性行为,这对于理解细胞组织至关重要.
科学领域:
- 生物物理学的生物物理.
- 软物质物理学 软物质物理学
- 分子生物学分子生物学
背景情况:
- 生物分子凝聚物具有不同的粘弹性特性,受分子序列和组成的影响.
- 固有无序蛋白质 (IDP) 的粗粒度模型对于研究凝聚物结构和热力学至关重要.
- 现有的灵活链模型往往无法捕捉复杂的粘性弹性,只能预测粘性行为.
研究的目的:
- 开发一个粗粒度模型,准确地复制实验性特征的凝结物的粘弹性特性.
- 调查依赖序列的链条刚性的作用,以确定凝结物的粘性弹性.
- 为了建立一个普遍的相关性,以频率依赖的损失因子在IDP冷凝液.
主要方法:
- 开发粗粒度分子模型,将依赖序列的链性纳入内在无序的蛋白质.
- 模拟和分析由A1-LCD及其突变体形成的冷凝物.
- 计算弹性和粘度模块以及频率依赖的损失因子.
- 链条刚性,旋转半径和粘弹性特性之间的相关性分析.
主要成果:
- 具有序列依赖链条刚性的模型准确地复制了A1-LCD凝结物的实验弹性和粘性模块.
- 一个单一的参数在各种序列和模型变化中普遍将依赖频率的损失因子与粘度相关联.
- 链条刚度增加,由更大的旋转半径证明,扩大了凝结物的弹性状态.
- 序列重新排列促进贴纸集群的形成被证明是调整粘性弹性.
结论:
- 依赖序列的链条刚性对于准确建模生物分子凝聚物的粘弹性行为至关重要.
- 该研究为理解和预测内在无序蛋白质凝聚物的序列编码粘弹性提供了一个框架.
- 这种方法提供了对材料性质的微观起源和它们在细胞区域中的调节的洞察.
相关概念视频
Relation between Poisson's ratio, Modulus of Elasticity and Modulus of Rigidity
332
Deformation occurs in axial and transverse directions when an axial load is applied to a slender bar. This deformation impacts the cubic element within the bar, transforming it into either a rectangular parallelepiped or a rhombus, contingent on its orientation. This transformation process induces shearing strain. Axial loading elicits both shearing and normal strains. Applying an axial load instigates equal normal and shearing stresses on elements oriented at a 45° angle to the load axis.
332
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration
1.7K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
According to Hooke's law, the vibrational frequency is directly proportional to...
1.7K
Mechanisms of Membrane-bending
2.8K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
2.8K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
1.2K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.2K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
920
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
920
Adaptability of Cytoskeletal Filaments
3.9K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.9K


