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Updated: Sep 15, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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马格伦 - - 一个可扩展的平台,用于冷EM数据可视化,管理和处理

Behdad Khoshbin1, Puneeth Damodar1, Rupali R Garje2

  • 1Institute of Molecular Biophysics, Florida State University; Tallahassee, FL, USA.

bioRxiv : the preprint server for biology
|July 15, 2025
PubMed
概括

马杰伦是单粒子冷电子显微镜 (cryo-EM) 的新软件平台,简化了数据处理和分析. 它提供了一个可扩展的微服务架构,用于高效的宏分子结构确定.

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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