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主要瘤微生物组预测结直肠癌的远程转移

Bishal Parajuli, Vishal Midya, Ryan Kiddle

bioRxiv : the preprint server for biology

|July 15, 2025

概括

主要瘤微生物组组成可以预测结直肠癌 (CRC) 转移. 像B. fragilis和E. coli这样的特定细菌与转移风险有关,提供潜在的预后标志物.

科学领域:

在瘤学瘤学.
微生物组研究微生物组研究
基因组学就是基因组学.

背景情况:

转移是结直肠癌 (CRC) 死亡的主要原因.
初级瘤中的微生物组变化可能对CRC转移具有预后意义.

研究的目的:

调查原发性瘤微生物组对于远程CRC转移的预测价值.
为了确定与无转移生存相关的特定微生物特征.

主要方法:

从ORIEN队列中对900个CRC瘤微生物组 (RNAseq) 的回顾性分析.
在一个独立的队列中使用16S rDNA测序和病原生物特异性qPCR进行验证.
评估微生物组的α和β多样性,以及微生物共发生的模式.

主要成果:

微生物组α多样性在初级瘤中较高,与转移风险相关.
微生物群的β多样性将原发性瘤与转移区分开来,并预测了无转移的5年生存期.
特定的细菌,包括高B. fragilis和低F. nucleatum,以及转移中的丰富的Enterobacteriaceae (大肠杆菌),与MFS有关. 肠毒性B. fragilis和pks+大肠杆菌在转移患者中增加. 一个3个物种的微生物群预测了转移 (OR 1.9).

结论:

主要瘤微生物组作为结直肠癌转移风险的精确标记.
特定的病原生物及其丰度模式是预测CRC转移的关键指标.

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