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相关概念视频

Protein Glycosylation01:25

Protein Glycosylation

7.3K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
7.3K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.0K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.0K

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相关实验视频

Updated: Sep 15, 2025

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

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N-甘氨基百科:本地甘氨基的图书馆 N-甘氨基结构分析

Christopher Ashwood1,2,3, Richard D Cummings1

  • 1Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, United States.

bioRxiv : the preprint server for biology
|July 15, 2025
PubMed
概括

这项研究介绍了N-甘氨酸百科,这是226个N-甘氨酸标准的全面资源. 这个N-甘氨酸数据库提供了验证的结构数据,以改善甘氨酸研究.

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Glycan Node Analysis: A Bottom-up Approach to Glycomics
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Glycan Node Analysis: A Bottom-up Approach to Glycomics

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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+

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相关实验视频

Last Updated: Sep 15, 2025

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
11:21

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Glycan Node Analysis: A Bottom-up Approach to Glycomics
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Glycan Node Analysis: A Bottom-up Approach to Glycomics

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Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
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科学领域:

  • 格莱科米克斯 (Glycomics) 是一个有趣的药物.
  • 生物化学 生物化学
  • 分析化学 分析化学

背景情况:

  • 由于结构多样性和缺乏全面标准,甘氨酸蛋白N-甘氨酸分析具有挑战性.
  • 现有的方法通常依赖于组成数据或预测结构,限制了详细的甘氨酸分析.

研究的目的:

  • 开发一个强大的N-甘氨酸分析和结构赋值平台.
  • 创建一个集中的资源,称为N-glycopedia,用于N-glycan研究.

主要方法:

  • 使用了226个不同的N-甘氨酸标准 (寡甘,混合,复杂类型) 的库.
  • 采用多孔石墨化碳 (PGC) 液态染色学-质谱学 (LC-MS) 用于高分辨率分离未衍生N-糖.
  • 生成的染色图库,包含保留时间,诊断片段和验证的结构赋值.

主要成果:

  • 通过使用PGC-LC-MS成功地解决和表征了一组多样化的226个N-甘氨酸.
  • 建立了一个全面的N-glycopedia资源,包含对N-glycans的验证结构数据.
  • 展示了该平台对针对性和基于发现的糖性化学品的能力.

结论:

  • 该N-甘氨基百科为克服当前N-甘氨酸分析的局限性提供了一个至关重要的资源.
  • 在PGC-LC-MS方法提供高分辨率的未衍生N-甘氨酸结构.
  • 这种可扩展的资源将通过实现准确的结构比较来推动糖化学研究.