慢性压力对全血转录组的影响:从动物模型中对公开可用的数据集的元分析
Elizabeth I Flandreau1, Duy Manh Nguyen2, Megan H Hagenauer3
1Grand Valley State University, Allendale, MI 49401 USA.
bioRxiv : the preprint server for biology
|July 15, 2025
概括
慢性压力显著改变了小鼠血液中的基因表达,影响了免疫反应和细胞衰老. 这项研究强调了血液转录组作为在压力研究中桥梁人类和动物模型的宝贵工具.
科学领域:
- 基因组学就是基因组学.
- 神经科学是一个神经科学.
- 免疫学 免疫学 免疫学
背景情况:
- 慢性压力对人类神经精神疾病有风险.
- 动物模型对于理解压力引起的变化和改善诊断/治疗至关重要.
- 跨物种比较研究需要统计能力和标准化结果测量.
研究的目的:
- 为了研究慢性压力对小鼠全血转录组的影响.
- 为了确定与慢性压力暴露相关的特定基因表达模式.
- 探索血液转录组作为人类和动物模型之间的翻译桥梁的实用性.
主要方法:
- 在三个公开的小鼠转录基因数据集 (GSE68076,GSE72262,GSE84185) 上进行了元分析.
- 鼠被暴露在慢性社会失败或慢性轻度压力范式中.
- 对9,219个基因进行了差异基因表达分析和功能丰富分析.
主要成果:
- 总共有39个转录被显著改变 (FDR<0.05),其中23个下调和16个上调.
- 低调基因组包括那些涉及B细胞功能,免疫反应,DNA/染色质调节和细胞代谢的基因组.
- 上调的基因组主要与红细胞和氧结合有关.
结论:
- 慢性压力会诱导小鼠血液中的分子变化,包括免疫失调.
- 这些发现支持了环境压力加速细胞衰老的假设.
- 小鼠血液转录组作为人类应激反应的相关模型.
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