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在接种疫苗和感染猪的流感特异性抗体介导和补充依赖的细胞细胞毒性诱导抗体

Mithilesh Singh¹, Gabriela Mansano do Nascimento², Sankar Renu¹

¹Center for Food Animal Health, Department of Animal Sciences, College of Food, Agricultural, and Environmental Sciences, The Ohio State University, Wooster, OH, United States.

Frontiers in immunology

|July 15, 2025

概括

对猪的抗体依赖性细胞毒性 (ADCC) 和补体依赖性细胞毒性 (CDC) 的优化试验显示,接种途径和类型影响了对猪流感A病毒 (SwIAV) 的免疫反应.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
疫苗学疫苗学疫苗学

背景情况:

抗体通过中和,ADCC和CDC提供保护.
猪是人类流感研究的一个有价值的模型.
现有有限的工具可以研究ADCC/CDC对猪的SwiAV的研究.

研究的目的:

在猪中优化ADCC和CDC对Swiav的测定.
评估不同SwIAV疫苗的抗体介导细胞毒性.
根据路线,类型和辅助剂来比较疫苗的疗效.

主要方法:

优化了ADCC和CDC的测定方法.
用鼻内基托纳米颗粒基失活化单价和肌内商用多价SWIAV疫苗接种疫苗的猪.
量化和比较抗体介导的细胞毒性.

主要成果:

母性抗体阳性猪在接种疫苗后未能引起ADCC抗体,但产生了CDC抗体.
挑战感染引起了高水平的ADCC抗体.
疫苗接种路线,疫苗类型和辅助剂影响了抗体功能.

结论:

测量ADCC和CDC抗体对于评估猪中SwiAV疫苗诱导的免疫力至关重要.
监测中和非中和抗体有助于开发交叉保护性疫苗.
优化的测试为未来的Swiav疫苗开发和评估提供了工具.

关键词:

在ADCC和CDC的研究中,抗体功能抗体功能基托桑纳米颗粒是一种纳米粒子.内接种疫苗内接种疫苗猪流感是一种病毒.

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Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.