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圆形RNA的微阵列概况确定CBT15_circR_28491和T辅助细胞是深静脉血栓形成的新调节者

Weiwei Chen¹, Ying Zhu¹, Sihua Niu¹

¹Department of Nursing, Shanghai General Hospital, Shanghai Jiao Tong University School of Nursing, Shanghai, China.

Frontiers in cardiovascular medicine

|July 15, 2025

概括

这项研究揭示了关键的循环RNA (circRNA) 和信使RNA (mRNA) 参与深静脉血栓症 (DVT) 的进展. 这些发现突出了一个特定的circRNA-miRNA-hub基因网络作为潜在的治疗目标DVT.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

深静脉血栓症 (DVT) 是一种严重的心血管疾病,死亡率和发病率很高.
了解DVT的分子和免疫格局对于开发有效的治疗方法至关重要.

研究的目的:

在深静脉血栓症 (DVT) 中识别差异表达的循环RNA (circRNA) 和信使RNA (mRNA).
阐明与DVT进展相关的分子和免疫特征.
在DVT中构建circRNAs,microRNAs (miRNAs) 和枢纽基因的调控网络.

主要方法:

使用GSE148333数据集上的limma包对circRNAs和mRNAs的差异表达分析.
权重基因同表达网络分析 (WGCNA) 来识别与DVT相关的模块和基因.
使用多个算法 (MCC,MNC,EPC,DEGREE) 进行枢纽基因鉴定,并构建一个circRNA-miRNA-枢纽基因网络.
免疫细胞透和与枢纽基因相关性的分析,在DVT大鼠模型中使用实时定量PCR进行验证.

主要成果:

在DVT中鉴定了421个差异表达的circRNA和1082个差异表达的mRNA.
发现了235个circRNA和207个mRNA与DVT相关,富含NOD类受体和mTOR信号传递等途径.
确定了17个关键的枢纽基因,包括*Birc5*,*Plk4*和*Kif18a*,并构建了一个circRNA-miRNA-枢纽基因网络,特别是CBT15_circR_28491-rno-miR-139-3p-Kif18a/Cdca8/Nek2.
观察到八种免疫细胞类型的差异性透,其中T辅助细胞与所有17个枢纽基因正相关.

结论:

这项研究为DVT中circRNA和mRNA的分子和免疫配置提供了关键的见解.
已确定的circRNA-miRNA-hub基因网络,特别是CBT15_circR_28491-rno-miR-139-3p-Kif18a/Cdca8/Nek2,代表了对DVT治疗的有希望的治疗目标.

关键词:

环RNAmiRNA枢纽基因网络圆形的RNAs是圆形的RNAs.深静脉血栓形成的原因是深静脉血栓形成.核心基因核心基因核心基因免疫细胞免疫细胞

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