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关于埃及乌 (Testudo kleinmanni) 语言形态学的新见解使用免疫光分析

Samir A A El-Gendy¹, Mohamed A M Alsafy¹, Ahmed A El-Mansi²

¹Anatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Abis 10th P.O. 21944, Alexandria, Egypt.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

|July 15, 2025

概括

这项研究揭示了对埃及海舌头解剖学的新见解,确定了味蕾分布和像维丁和内斯这样的分子标记. 这些发现增强了我们对这种物种的理解.

科学领域:

兽医医学兽医医学兽医医学
比较解剖学的比较解剖学
细胞生物学细胞生物学

背景情况:

埃及海 (Testudo kleinmanni) 是一种具有独特适应性的物种.
关于味蕾的分布及其舌头细胞的分子特征的知识有限.

研究的目的:

为了研究埃及海舌头上的味蕾的分布和细胞组成.
在Testudo kleinmanni中表征舌细胞的分子标记物.
为了解埃及海的生理适应提供见解.

主要方法:

使用六种特定抗体进行免疫光.
光显微镜. 光显微镜.
扫描电子显微镜. 扫描电子显微镜.

主要成果:

在语言体中发现了表面的味蕾,比语言根中的味蕾更大.
在语言根的真菌状乳头中发现了深层的味蕾.
维门在所有味蕾细胞中得到了广泛的表达,而内斯在基底细胞中得到了特别的表达. PDGFRα和CD34的共同表达表明了类似于三角细胞的细胞. CD19确定了B细胞淋巴细胞.
观察到质软骨,与PDGFRα和SOX9共同表达在冠状细胞,SOX9是更高的表达.

结论:

这项研究是首次将免疫光应用于埃及海的舌头,揭示了其味蕾的新型结构和分子细节.
这些发现有助于更好地了解物种的生物学及其生理适应.

关键词:

埃及海是埃及海的一种.这是NestinNestin的巢穴.免疫光效应免疫光效应味蕾味蕾味蕾味蕾舌头舌头舌头舌头它们的味道很好.

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相关概念视频

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.