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Updated: Sep 15, 2025

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用马雷地图方法绘制与增长相关的亚洲海SNP标记的映射 (最新的)

Permkullaya Yuttiwat¹, Sila Sukhavachana², Naruechon Pattarapanyawong³

¹Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand.

Marine biotechnology (New York, N.Y.)

|July 15, 2025

概括

这项研究开发了一个基于SNP的亚洲海链接地图,识别标志物-生长特征关联. 这张地图有助于未来的基因研究,以改善鱼类生长特征.

科学领域:

水产养殖遗传学水产养殖遗传学
基因组学就是基因组学.
鱼类繁殖鱼类繁殖

背景情况:

亚洲海 (Lates calcarifer) 是一个经济上重要的水产养殖物种.
了解影响生长的遗传因素对于选择性育种计划至关重要.
高密度遗传图对于定量特征位置 (QTL) 绘制至关重要.

研究的目的:

为了研究亚洲海的标志物-生长特征关联.
为泰国KU人口构建基于单核酸多态 (SNP) 的遗传链接地图.
为未来的遗传分析开发具有竞争力的基因特异性PCR (KASP) 标记物.

主要方法:

基因组广泛关联研究 (GWAS) 在1100只鱼中使用表型 (体重,长度) 和基因型 (25,647个SNP) 进行.
使用Marey地图方法预测了基因链接地图,使用现有的全基因组SNP数据和KU人口数据.
竞争性等位基特异性PCR (KASP) 标志物从显著的SNP中开发并验证.

主要成果:

使用GBLUP,GWAS确定了70个与重量和长度相关的显著SNP,其繁殖价值预测准确度高 (0.650.70).
为KU群体构建了一个基于SNP的链接地图,跨越24个染色体 (567 Mb),平均标记间距为0.07 cM.
针对70个SNP成功开发了KASP测定,其中58个在整个基因组中分离,提供了宝贵的资源.

结论:

该研究成功地为亚洲海KU种群构建了遗传链接地图.
已识别的标记-生长特征关联为标记辅助选择提供了基础.
开发的链接地图和KASP标记器将促进未来的QTL绘制与生长相关的特征.

关键词:

在GWAS中,GWAS就是GWAS.卡斯普 (KASP) 公司链接地图链接地图标记效应的标记效应是指标效应的影响.重组率是指重组率是指重组率.

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Genome-wide Association Studies-GWAS

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Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
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A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...