相关实验视频
Updated: Sep 15, 2025

08:22
Studying Copper Nanoparticle-Induced Programmed Cell Death in Bacteria
Published on: May 16, 2025
242
神经生长因子在铜诱导的肝损伤中作为p38 MAPK通路的调节器
Mustafa Usta1, Yılmaz Çiğremiş2, Hasan Özen1
1Department of Pathology, Faculty of Veterinary Medicine, Balıkesir University, Balıkesir 10100, Turkiye.
概括
神经生长因子 (NGF) 通过p38 MAPK通路减少氧化应激和炎症,保护肝脏免受铜毒性影响. 这项研究突出了NGF的重点.
科学领域:
- 肝病学和毒理学 肝病学和毒理学
- 分子和细胞生物学分子和细胞生物学
- 神经科学和信号通路的神经科学和信号通路
背景情况:
- 铜 (Cu) 毒性会在肝细胞 (肝细胞) 中引发氧化和化应激,导致炎症和编程细胞死亡 (亡).
- 神经生长因子 (NGF) 已被认为具有神经保护作用,并可能通过p38基激活蛋白激酶 (MAPK) 途径影响肝脏组织.
- 在此之前,NGF在减轻铜诱导的肝损伤方面的确切作用尚不清楚.
研究的目的:
- 在铜 (Cu) 诱导的肝损伤的小鼠模型中研究外源神经生长因子 (NGF) 的潜在肝保护作用.
- 阐明p38 MAPK信号通路参与NGF对Cu肝毒性的保护机制.
主要方法:
- 64只雄性BALB/c小鼠被分配到八个实验组,包括对照组,NGF,p38 MAPK抑制剂 (SB203580),以及与硫酸铜 (CuSO4) 的各种组合.
- 药物通过24小时间隔的三次腹腔内注射进行了药物注射.
- 肝脏组织被评估使用组织病理学,免疫组织化学,生物化学和分子分析来评估损伤和细胞反应.
主要成果:
- 硫酸铜 (CuSO4) 暴露导致严重的肝损伤,其特征是水性退行,亡,亡,肝酶升高 (ALT/AST),氧化/化应激标志物增加.
- 使用神经生长因子 (NGF) 显著减少肝损伤,改善抗氧化能力 (GSH,TAC),并抑制促炎细胞因子 (IL-1,IL-6,TNF-α).
- 当与p38 MAPK抑制剂 (SB203580) 联合使用时,NGF的保护作用被废除,证实了该途径的关键作用.
结论:
- 神经生长因子 (NGF) 显示出对抗铜诱导的肝损伤的显著肝保护能力.
- NGF通过调节氧化应激,炎症和亡,主要通过p38 MAPK信号通路来减轻Cu的毒性.
- 这些发现表明NGF在治疗与氧化应激相关的肝损伤方面具有治疗潜力.
相关概念视频
Interactions Between Signaling Pathways
6.5K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.5K
Liver Regeneration
3.4K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.4K
NF-κB-dependent Signaling Pathway
7.9K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.9K
MAPK Signaling Cascades
6.1K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
6.1K

