微生物介导的芬顿系统的协同增强机制在草堆肥过程中对素脱聚合作用
Fengting Qu1, Li Zhao2, Yinan Cao3
1Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, China; College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
Bioresource technology
|July 15, 2025
概括
这项研究使用微生物芬顿系统在堆肥中增强了氨酸脱聚合. 结合的细菌,真菌和硫酸铁 (FeSO4) 通过优化活性氧物种 (ROS) 和酶活性,显著促进了素分解.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 生物化学 生物化学
背景情况:
- 素脱聚合对于农业废物回收和堆肥至关重要.
- 微生物介导的芬顿系统为高效的素降解提供了一个有希望的方法.
- 了解协同机制是优化堆肥技术的关键.
研究的目的:
- 研究微生物介导的芬顿系统在素脱聚合中的协同增强机制.
- 评估细菌,真菌和硫酸铁 (FeSO4) 对素降解的联合作用.
- 阐明反应性氧物种 (ROS) 和酶活动在这个过程中的作用.
主要方法:
- 使用微生物产生的过氧化 (H2O2) 和Fe (II) 构建了芬顿系统.
- 实施了四种治疗方法:控制 (CK),细菌-真菌注射 (BHF),FeSO4添加 (Fe) 和与FeSO4 (BHF-Fe) 联合注射.
- 评估了酶活性 (乳酶,红素过氧化酶),ROS生成 (超氧化离子,H2O2,基),红素损失率和基因表达 (Lac,LiP,MnP).
主要成果:
- 与对照组相比,BHF-Fe处理显示了22.58%的较高的红素损失率.
- 同步的细菌和真菌注射优化了纤维素和纤维素降解率.
- 添加FeSO4进一步优化了基 (·OH) 生产和ROS生成,增强了与素损失的相关性.
- 通过对关键降解酶基因的上调加速了宁分解.
- 芬顿系统表现出减弱的pH依赖.
结论:
- 涉及芬顿反应,ROS,生物催化酶氧化和微环境补偿的协同机制推动了高效的素降解.
- 微生物介导的芬顿系统,特别是BHF-Fe,为农业废物回收和堆肥提供了一个强大的战略.
- 这项研究为推进堆肥技术提供了理论和技术支持.
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